A novel NK-lysin in hybrid crucian carp can exhibit cytotoxic activity in fish cells and confer protection against Aeromonas hydrophila infection in comparison with Carassius cuvieri and Carassius auratus red var
| Autor: | Sheng-Wei Luo, Shi Wang, Lanfen Fan, Fangzhou Hu, Kaikun Luo, Ning-Xia Xiong, Chang Wu, Ming Wen, Zhuang-Wen Mao, Zi-Ye Luo, Shaojun Liu |
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| Rok vydání: | 2021 |
| Předmět: |
Fish Proteins
Lipopolysaccharides 0301 basic medicine Carps Lipopolysaccharide Cell Survival Proteolipids Gene Expression Spleen Aquatic Science Biology Kidney complex mixtures Fish Diseases 03 medical and health sciences chemistry.chemical_compound medicine Animals Environmental Chemistry Cytotoxic T cell RNA Messenger Viability assay Cells Cultured Membrane Potential Mitochondrial Chimera Effector 04 agricultural and veterinary sciences General Medicine biology.organism_classification Molecular biology Aeromonas hydrophila 030104 developmental biology medicine.anatomical_structure Liver chemistry Cell culture Animal Fins 040102 fisheries Crucian carp bacteria 0401 agriculture forestry and fisheries Gram-Negative Bacterial Infections Reactive Oxygen Species |
| Zdroj: | Fish & Shellfish Immunology. 116:1-11 |
| ISSN: | 1050-4648 |
| Popis: | NK-lysin, an effector of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), not only exhibits cytotoxic effect in fish cells, but also participates in the immune defense against pathogenic infection. In this study, ORF sequences of RCC-NK-lysin, WCC-NK-lysin and WR-NK-lysin were 369 bp. Tissue-specific analysis revealed that the highest expressions of RCC-NK-lysin and WCC-NK-lysin were observed in gill, while the peaked level of WR-NK-lysin mRNA was observed in spleen. A. hydrophila infection sharply increased RCC-NK-lysin, WCC-NK-lysin and WR-NK-lysin mRNA expression in liver, trunk kidney and spleen. In addition, elevated levels of NK-lysin mRNA were observed in cultured fin cell lines of red crucian carp (RCC), white crucian carp (WCC) and their hybrid offspring (WR) after Lipopolysaccharide (LPS) challenge. RCC-NK-lysin, WCC-NK-lysin and WR-NK-lysin exerted regulatory roles in inducing ROS generation, modulating mitochondrial membrane potential, decreasing fish cell viability and antagonizing survival signalings, respectively. RCC/WCC/WR-NK-lysin-overexpressing fish could up-regulate expressions of inflammatory cytokines and decrease bacterial loads in spleen. These results indicated that NK-lysin in hybrid fish contained close sequence similarity to those of its parents, possessing the capacities of cytotoxicity and immune defense against bacterial infection. |
| Databáze: | OpenAIRE |
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