Application of probe electrospray ionization-tandem mass spectrometry to ultra-rapid determination of glufosinate and glyphosate in human serum
| Autor: | Haruka Kobayashi, Masato Funayama, Yoshito Kamijo, Eriko Minami, Tomoki Hanazawa, Eito Kubota, Kiyotaka Usui, Yuji Fujita, Tomohiro Yoshizawa |
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| Rok vydání: | 2019 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Calibration curve Electrospray ionization Clinical Biochemistry Glycine Pharmaceutical Science Tandem mass spectrometry 01 natural sciences Analytical Chemistry chemistry.chemical_compound Limit of Detection Tandem Mass Spectrometry Present method Drug Discovery Humans Derivatization Spectroscopy Chromatography High Pressure Liquid Detection limit Chromatography 010405 organic chemistry Herbicides Aminobutyrates 010401 analytical chemistry Solid Phase Extraction Reproducibility of Results Reference Standards 0104 chemical sciences chemistry Glufosinate Glyphosate Calibration Chromatography Liquid |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 174 |
| ISSN: | 1873-264X |
| Popis: | Glufosinate and glyphosate, which are non-selective herbicides that include an amino acid moiety in their structures, are frequently used worldwide to control unwanted vegetation. Unfortunately, these readily available herbicides are also used by people to commit suicide, and thus represent important chemicals of interest in the fields of clinical medicine and forensics. Because of the high water solubility of these herbicides, most analytical methods for their detection require a derivatization step, which results in longer analysis times. Therefore, derivatization-based methods do not currently contribute to judgements on treatment decisions in emergency medicine. In this study, we addressed this limiting factor by developing an ultra-rapid and simple analytical technique using a combination of probe electrospray ionization (PESI) and tandem mass spectrometry (MS/MS), which gives quantitative results within 0.3 min. Herbicide standards were added to human serum that was then subjected to analysis (N = 5 per concentration). The analysis was repeated daily over eight consecutive days. The limit of detection (LOD) was 0.59 μg/mL for glufosinate and 0.20 μg/mL for glyphosate. The limit of quantitation (LOQ), i.e., the lowest point on the calibration curves, was 1.56 μg/mL for both the herbicides. The matrix effects were observed at three different concentrations (between 95.7%–104% for glufosinate, and between 90.7%–95.7% for glyphosate). When applied to samples taken from actual poisoning cases (six samples for each herbicide), the present method gave almost the same quantitative values as those obtained by conventional high-performance liquid chromatography with fluorescence detection. Thus, we believe that PESI-MS/MS could emerge as a rapid diagnosis method in the clinical emergency field. |
| Databáze: | OpenAIRE |
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