Inactivation of H‐type and L‐type bovine spongiform encephalopathy following recommended autoclave decontamination procedures

Autor:	Sarah R. Walker, Peter C. Griffiths, Jemma K. Thorne, Marion Simmons, Katy E. Beck, Christopher M. Vickery, Gail E. Chapman, Linda A. Terry, John Spiropoulos, Cristina Casalone, Richard Lockey, Leigh Thorne, Lucien van Keulen, Mark Arnold
Rok vydání:	2020
Předmět:	040301 veterinary sciences animal diseases Bovine spongiform encephalopathy transmissible spongiform encephalopathie H-type Autoclave Incubation period 0403 veterinary science 03 medical and health sciences mental disorders medicine Bioassay inactivation bovine spongiform encephalopathy L-type 030304 developmental biology Host Pathogen Interaction & Diagnostics Infectivity 0303 health sciences General Veterinary General Immunology and Microbiology Chemistry Bacteriologie food and beverages Bacteriology Bacteriology Host Pathogen Interaction & Diagnostics 04 agricultural and veterinary sciences General Medicine Human decontamination medicine.disease Virology Host Pathogen Interactie & Diagnostiek nervous system diseases Titer Specified risk material Bacteriologie Host Pathogen Interactie & Diagnostiek
Zdroj:	Transboundary and Emerging Diseases 67 (2020) 5 Transboundary and Emerging Diseases, 67(5), 1872-1878
ISSN:	1865-1682 1865-1674
DOI:	10.1111/tbed.13513
Popis:	The resistance of H-type and L-type BSE prions to autoclaving under EU regulation conditions for specified risk material is unknown. We employed transgenic mouse (bovinized line tg1896) bioassay to assess the efficacy of such decontamination on L- and H-type BSE. For each source, titre calculation was based on the comparison of incubation period and attack rate prior to and after decontamination. The infectious titre of L-type BSE was reduced by at least 9.40 log10 and of H-type BSE by at least 3.94 log10. In fact, no infectivity was detected for L-type or H-type BSE post-inactivation even at a 10–1 dilution.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c33c3ac38590cf45330a983fc4dfe5ec https://doi.org/10.1111/tbed.13513 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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