Effect of excessive nitrogen on levels of amino acids and sugars, and differential response to post-harvest cold storage in potato (Solanum tuberosum L.) tubers
| Autor: | Zhuqing Zhao, Xinwei Liu, Haiqing Zhang, Bihua Nie, Wei Zhang, Botao Song |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sucrose Nitrogen Physiology Metabolite Cold storage chemistry.chemical_element Plant Science 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Nutrient Genetics Asparagine Food science Amino Acids Solanum tuberosum chemistry.chemical_classification Chemistry fungi food and beverages Amino acid Cold Temperature Plant Tubers 030104 developmental biology Sugars 010606 plant biology & botany |
| Zdroj: | Plant Physiology and Biochemistry. 157:38-46 |
| ISSN: | 0981-9428 |
| Popis: | Nitrogen (N) is an important nutrient for increased potato tuber yield. However, excessive N can decrease tuber quality. Furthermore, the impact of optimal and higher N levels of potato tuber metabolic profile at harvest and cold storage remains unclear. This study aimed to investigate the metabolic profiling of free amino acids and sugars in potato tubers affected by different nitrogen levels (optimal, ON; and excessive, EN) at harvest (AH) and cold storage (CS) (~4 °C, 4 weeks) through untargeted GC-TOF-MS, and targeted UHPLC-QqQ-MS. Carbohydrate content and vacuolar invertase activity (IV) were determined. Principal component analysis of metabolite data indicated a distinct separation between ON and EN treatments at harvest and cold storage. Multivariate data analysis revealed that sucrose, reducing sugars, and free asparagine were the most altered metabolites (VIP 1 and P 0.05), which were involved in starch and sucrose metabolism, and alanine, aspartate and glutamate metabolism. At harvest, the absolute contents of various free amino acids including asparagine were higher (by 1.3-1.5 fold) in the EN treatment than ON treatment, and this difference was maintained at 4-week cold storage. Under the EN treatment, tuber maturity was reduced, and sucrose accumulation was increased at harvest, while IV was increased after cold storage, reducing sugar also accumulated. These results highlighted the negative effects of EN on free amino acid and sugars metabolism in the post-harvest tubers and provided useful information for understanding the underpinning physiological mechanisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect