Establishment of a time‐resolved immunoassay for acute kidney injury based on the detection of Kim‐1
| Autor: | Zheng Shaoxiong, Xiumei Zhou, Yuan Qin, Yu Xiaomei, Chen Lingli, Liu Xiaobin, Yigang Wang, Gong Jianguang, Shen Shuijuan, Biao Huang |
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| Rok vydání: | 2022 |
| Předmět: |
Immunoassay
Serum Microbiology (medical) Fluoroimmunoassay Biochemistry (medical) Clinical Biochemistry Public Health Environmental and Occupational Health Hematology Acute Kidney Injury Immunologic Tests Medical Laboratory Technology Humans Immunology and Allergy Hepatitis A Virus Cellular Receptor 1 Biomarkers |
| Zdroj: | Journal of Clinical Laboratory Analysis. 36 |
| ISSN: | 1098-2825 0887-8013 |
| DOI: | 10.1002/jcla.24603 |
| Popis: | To establish a highly sensitive time-resolved fluorescence immunoassay (TRFIA) of kidney injury molecule-1 (Kim-1) and evaluate its clinical value in acute kidney injury (AKI).The Kim-1-TRFIA was established by the double-antibody sandwich method, and the method was evaluated. The established Kim-1-TRFIA was used to detect the concentration of Kim-1 in the serum of healthy controls and patients with AKI.The optimal coating antibody concentration and optimal EuA highly sensitive Kim-1-TRFIA was established. With this immunoassay, a good differential diagnosis can be made, and healthy people and AKI patients can be differentiated by detecting the concentration of Kim-1 in the serum. Moreover, the severity of AKI patients can be determined. |
| Databáze: | OpenAIRE |
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