Coding strategy of the S genome segment of Hantaan virus
| Autor: | John Hay, Joel M. Dalrymple, Connie S. Schmaljohn, Gerald B. Jennings |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Orthohantavirus
Genes Viral viruses Viral Proteins Capsid Virology Complementary DNA Amino Acid Sequence Cloning Molecular Gene Polymerase Hantaan virus Genetics Base Sequence biology Viral Core Proteins Nucleic acid sequence Chromosome Mapping virus diseases RNA DNA Restriction Enzymes biology.organism_classification Open reading frame Genes biology.protein Nucleic Acid Conformation RNA Viral Bunyaviridae |
| Zdroj: | Virology. 155:633-643 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(86)90223-0 |
| Popis: | Hantaan virus is the type species of the recently recognized Hantavirus genus of Bunyaviridae. The small (S) RNA segment of the negative-sense, tripartite genome was molecularly cloned and the nucleotide sequence was determined. The RNA sequence derived from the cDNA copy was found to contain 1696 nucleotides. A single open reading frame of sufficient size to encode the virus nucleocapsid protein was detected in the cDNA corresponding to viral complementary-sense RNA. RNA transcripts of the cDNA were synthesized with SP6 polymerase and were used to program cell-free reticulocyte lysate translation systems. Viral complementary-sense transcripts served as efficient messages in translation systems and generated Hantaan nucleocapsid protein. No translation products were detected when lysates were programmed with viral-sense transcripts. This coding assignment of the nucleocapsid protein to the viral complementary-sense RNA of the S genome segment is consistent with those of other members of this family. Unlike other Bunyaviridae, which encode both a nucleocapsid protein and a nonstructural (NS S ) protein of similar sizes, a NS S protein has not been identified for Hantaan virus. Furthermore, other than the nucleocapsid protein gene sequence, the only potential open reading frame in Hantaan S RNA encoded a short, 48-amino acid polypeptide which initiated two codons beyond the termination of the nucleocapsid protein in the same reading frame. These data demonstrate that the coding strategy of the Hantaan virus S RNA is different than those reported for other viruses in this family. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|