Vitamin D metabolism and profiling in veterinary species
| Autor: | Natalie Z.M. Homer, Emma Hurst, Richard J. Mellanby |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Veterinary medicine 040301 veterinary sciences Endocrinology Diabetes and Metabolism Metabolite lcsh:QR1-502 Single sample vitamin D Review Biology Biochemistry lcsh:Microbiology 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound Vitamin D+Metabolites free vitamin D Liquid chromatography–mass spectrometry Lc ms ms Vitamin D and neurology C3-epimers LC-MS/MS Molecular Biology Vitamin D metabolism comparative 04 agricultural and veterinary sciences veterinary Clinical Practice 030104 developmental biology 25-hydroxyvitamin-D 1 25-dihydroxyvitamin-D chemistry 24 25-dihydroxyvitamin-D profiling |
| Zdroj: | Hurst, E, Homer, N Z M & Mellanby, R 2020, ' Vitamin D metabolism and profiling in veterinary species ', Metabolites, vol. 10, no. 9 . https://doi.org/10.3390/metabo10090371 Metabolites Metabolites, Vol 10, Iss 371, p 371 (2020) |
| DOI: | 10.3390/metabo10090371 |
| Popis: | The demand for vitamin D analysis in veterinary species is increasing with the growing knowledge of the extra-skeletal role vitamin D plays in health and disease. The circulating 25-hydroxyvitamin-D (25(OH)D) metabolite is used to assess vitamin D status, and the benefits of analysing other metabolites in the complex vitamin D pathway are being discovered in humans. Profiling of the vitamin D pathway by liquid chromatography tandem mass spectrometry (LC-MS/MS) facilitates simultaneous analysis of multiple metabolites in a single sample and over wide dynamic ranges, and this method is now considered the gold-standard for quantifying vitamin D metabolites. However, very few studies report using LC-MS/MS for the analysis of vitamin D metabolites in veterinary species. Given the complexity of the vitamin D pathway and the similarities in the roles of vitamin D in health and disease between humans and companion animals, there is a clear need to establish a comprehensive, reliable method for veterinary analysis that is comparable to that used in human clinical practice. In this review, we highlight the differences in vitamin D metabolism between veterinary species and the benefits of measuring vitamin D metabolites beyond 25(OH)D. Finally, we discuss the analytical challenges in profiling vitamin D in veterinary species with a focus on LC-MS/MS methods. |
| Databáze: | OpenAIRE |
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