Multiplexed nanoplasmonic biosensor for one-step simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine

Autor: Maria Soler, Carole Kebbi-Beghdadi, Hatice Altug, Alexander Belushkin, Gilbert Greub, Andrea Cavallini
Rok vydání: 2017
Předmět:
DNA
Bacterial
Point-of-Care Systems
Microfluidics
Biomedical Engineering
Biophysics
Chlamydia trachomatis
02 engineering and technology
Biosensing Techniques
Biosensing Techniques/methods
Chlamydia Infections/urine
Chlamydia trachomatis/chemistry
Chlamydia trachomatis/isolation & purification
DNA
Bacterial/chemistry
DNA
Bacterial/isolation & purification
DNA
Bacterial/urine
Gonorrhea/microbiology
Gonorrhea/urine
Humans
Neisseria gonorrhoeae/chemistry
Neisseria gonorrhoeae/isolation & purification
Bacteria detection
Diagnosis
Multiplexing
Nanohole array
Nanoplasmonic biosensor
Sexually transmitted infections
Biology
medicine.disease_cause
01 natural sciences
Microbiology
Gonorrhea
Electrochemistry
medicine
Detection limit
Chromatography
medicine.diagnostic_test
010401 analytical chemistry
General Medicine
Chlamydia Infections
021001 nanoscience & nanotechnology
DNA extraction
Neisseria gonorrhoeae
0104 chemical sciences
Immunoassay
0210 nano-technology
Biosensor
Biotechnology
Zdroj: Biosensors & bioelectronics, vol. 94, pp. 560-567
ISSN: 1873-4235
Popis: Development of rapid and multiplexed diagnostic tools is a top priority to address the current epidemic problem of sexually transmitted diseases. Here we introduce a novel nanoplasmonic biosensor for simultaneous detection of the two most common bacterial infections: Chlamydia trachomatis and Neisseria gonorrhoeae. Our plasmonic microarray is composed of gold nanohole sensor arrays that exhibit the extraordinary optical transmission (EOT), providing highly sensitive analysis in a label-free configuration. The integration in a microfluidic system and the precise immobilization of specific antibodies on the individual sensor arrays allow for selective detection and quantification of the bacteria in real-time. We achieved outstanding sensitivities for direct immunoassay of urine samples, with a limit of detection of 300 colony forming units (CFU)/mL for C. trachomatis and 1500CFU/mL for N. gonorrhoeae. The multiplexing capability of our biosensor was demonstrated by analyzing different urine samples spiked with either C. trachomatis or N. gonorrhoeae, and also containing both bacteria. We could successfully detect, identify and quantify the levels of the two bacteria in a one-step assay, without the need for DNA extraction or amplification techniques. This work opens up new possibilities for the implementation of point-of-care biosensors that enable fast, simple and efficient diagnosis of sexually transmitted infections.
Databáze: OpenAIRE
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