Protective immunity to Japanese encephalitis virus associated with anti-NS1 antibodies in a mouse model
| Autor: | Dorian Counor, Vincent Deubel, Yize Li, Yongxin Yu, Peng Lu, Veasna Duong |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
viruses
T-cell response Antibodies Viral Nonstructural Proteins Antibodies Viral NS1 protein Cell Line Mouse model lcsh:Infectious and parasitic diseases Interferon-gamma Mice Immune system Adjuvants Immunologic Antigen Immunity Virology medicine Animals lcsh:RC109-216 Encephalitis Japanese Encephalitis Virus Japanese Antiserum Mice Inbred C3H Vaccines Synthetic biology Japanese Encephalitis Vaccines Research Viral encephalitis Immunization Passive virus diseases Japanese encephalitis biochemical phenomena metabolism and nutrition medicine.disease biology.organism_classification Disease Models Animal Flavivirus Japanese encephalitis virus Infectious Diseases Immunoglobulin G Leukocytes Mononuclear biology.protein Drosophila Female Monoclonal antibodies Antibody Spleen |
| Zdroj: | Virology Journal, Vol 9, Iss 1, p 135 (2012) Virology Journal |
| ISSN: | 1743-422X |
| DOI: | 10.1186/1743-422x-9-135 |
| Popis: | Background Japanese encephalitis virus (JEV) is a major mosquito-borne pathogen that causes viral encephalitis throughout Asia. Vaccination with an inactive JEV particle or attenuated virus is an efficient preventative measure for controlling infection. Flavivirus NS1 protein is a glycoprotein secreted during viral replication that plays multiple roles in the viral life cycle and pathogenesis. Utilizing JEV NS1 as an antigen in viral vectors induces a limited protective immune response against infection. Previous studies using E. coli-expressed JEV NS1 to immunize mice induced protection against lethal challenge; however, the protection mechanism through cellular and humoral immune responses was not described. Results JEV NS1 was expressed in and purified from Drosophila S2 cells in a native glycosylated multimeric form, which induced T-cell and antibody responses in immunized C3H/HeN mice. Mice vaccinated with 1 μg NS1 with or without water-in-oil adjuvant were partially protected against viral challenge and higher protection was observed in mice with higher antibody titers. IgG1 was preferentially elicited by an adjuvanted NS1 protein, whereas a larger load of IFN-γ was produced in splenocytes from mice immunized with aqueous NS1. Mice that passively received anti-NS1 mouse polyclonal immune sera were protected, and this phenomenon was dose-dependent, whereas protection was low or delayed after the passive transfer of anti-NS1 MAbs. Conclusion The purified NS1 subunit induced protective immunity in relation with anti-NS1 IgG1 antibodies. NS1 protein efficiently stimulated Th1-cell proliferation and IFN-γ production. Protection against lethal challenge was elicited by passive transfer of anti-NS1 antisera, suggesting that anti-NS1 antibodies play a substantial role in anti-viral immunity |
| Databáze: | OpenAIRE |
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