Use of ultrasound contrast agent microbubbles in preclinical research: recommendations for small animal imaging
| Autor: | Christophe Arbogast, Alexandre Helbert, Martine Theraulaz, Jean-Marc Hyvelin, François Tranquart, Isabelle Tardy, Maria Costa, Patricia Emmel, Adrian D. Nunn |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
media_common.quotation_subject Drug Evaluation Preclinical Contrast Media Preclinical research Mice Small animal High spatial resolution Medicine Contrast (vision) Animals Radiology Nuclear Medicine and imaging Medical physics media_common Ultrasonography Modality (human–computer interaction) Microbubbles business.industry Ultrasound General Medicine Image Enhancement Molecular Imaging Rats Molecular imaging business |
| Zdroj: | Investigative radiology. 48(8) |
| ISSN: | 1536-0210 |
| Popis: | Ultrasound contrast imaging techniques represent a real opportunity to improve efficiency in the preclinical drug discovery and development process. Ultrasound contrast agents (UCAs) combined with specific ultrasound contrast detection modes provide real-time, high spatial resolution of both organ and lesion blood perfusion, the so-called dynamic contrast-enhanced ultrasound imaging. With the advent of targeted UCA, ultrasound molecular imaging is gaining momentum in molecular imaging, particularly because of the simultaneous real-time anatomical and functional/molecular imaging capabilities. In preclinical research, contrast-enhanced ultrasound imaging, with either nontargeted or targeted UCA, is a fast-growing imaging modality that has not yet been standardized compared with other imaging modalities. Contrast-enhanced ultrasound imaging is an operator-dependent imaging modality, requiring adherence to rigorous step-by-step protocols. In this article, which is intended for advanced, hands-on researchers, we report key factors that can lead to variability in preclinical results and recommend some preventive methods to limit or cancel their effect on the final results. Standardized procedures are a prerequisite for acceptance of new contrast-enhanced ultrasound imaging methods to eliminate factors that could distort results, improve the reproducibility between different centers and studies, and, therefore, allow translation to clinical application. |
| Databáze: | OpenAIRE |
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