The human PD-1 gene: complete cDNA, genomic organization, and developmentally regulated expression in B cell progenitors
| Autor: | Lawrence R. Finger, Elaine Louie, Jaiyu Pu, L G Billips, Robert Wasserman, Richard R. Hardy, Rajeev Vibhakar, Peter D. Burrows |
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| Rok vydání: | 1997 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Programmed Cell Death 1 Receptor Restriction Mapping Locus (genetics) Regulatory Sequences Nucleic Acid Biology Homology (biology) Mice Exon Antigens CD Bone Marrow Sequence Homology Nucleic Acid Complementary DNA Genetics Animals Humans Amino Acid Sequence RNA Messenger Gene Cells Cultured Genomic organization chemistry.chemical_classification B-Lymphocytes Mice Inbred BALB C Base Sequence Sequence Homology Amino Acid Interleukin-7 Gene Expression Regulation Developmental Proteins General Medicine Hematopoietic Stem Cells Molecular biology Amino acid DNA binding site Genes chemistry Chromosomes Human Pair 2 Antigens Surface Female Apoptosis Regulatory Proteins |
| Zdroj: | Gene. 197:177-187 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/s0378-1119(97)00260-6 |
| Popis: | We report the complete cDNA sequence and the genomic structure of the human PD-1 homologue. An analysis of the expression pattern of the human PD-1 gene (hPD-1) and the murine PD-1 gene (mPD-1) in developing bone marrow B-lineage cells was also undertaken. The full length hPD-1 cDNA is 2106 nucleotides long and encodes a predicted protein of 288 amino acid residues. The hPD-1 and mPD-1 genes share 70% homology at the nucleotide level and 60% homology at the amino acid level. Four potential sites for N-linked glycosylation are conserved, as are a stretch of amino acids between two cysteine residues resembling a V-set immunoglobulin domain, and another region containing a motif similar to an immunoreceptor tyrosine-based inhibitory motif. Isolation of the genomic locus of the hPD-1 gene reveals that the gene is composed of five exons located on human chromosome 2 at band q37. The 5′ flanking region lacks TATA and CAAT cis-acting elements, but includes a number of potential transcription factor binding sites and a dominant transcription start site. The mPD-1 gene was preferentially expressed in pro-B cells from murine adult bone marrow. Although hPD-1 was not preferentially expressed in pro-B cells from human fetal bone marrow, treatment of isolated pro-B cells with interleukin-7 resulted in a dramatic increase in expression. These data suggest that PD-1 may play a role in B-cell differentiation during the pro-B cell stage. |
| Databáze: | OpenAIRE |
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