Compaction of Islets Is Detrimental to Transplant Outcome in Mice
| Autor: | A. M. James Shapiro, Shaheed Merani, Camillo Ricordi, Colin C. Anderson, Wayne Truong, Jonathan R. T. Lakey, Colleen D. Schur, Victor K. Knutzen |
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| Rok vydání: | 2006 |
| Předmět: |
endocrine system
medicine.medical_specialty endocrine system diseases Ratón medicine.medical_treatment Cell Culture Techniques Islets of Langerhans Transplantation Mice Inbred Strains Biology Islets of Langerhans Mice In vivo Internal medicine medicine Animals Insulin Glucose homeostasis Cells Cultured Mice Inbred BALB C Transplantation Glucose tolerance test geography geography.geographical_feature_category medicine.diagnostic_test Graft Survival Islet Endocrinology Apoptosis Tissue and Organ Harvesting |
| Zdroj: | Transplantation. 82:1472-1476 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/01.tp.0000243166.64244.3d |
| Popis: | Background. Despite recent progress in clinical islet transplantation, the cumulative world experience remains small. Optimizing protection of islets throughout the isolation, purification, and peritransplant period remains critical to outcome. We herein investigate the potential detrimental impact of maintaining islets in a pelleted state for periods preceding implantation. We hypothesize that periods of islet compaction lead to impairment if islet function in vivo. Methods. In this study, 250-islet marginal mass transplants were conducted in the BALB/c syngeneic mouse model using islets either preincubated as an islet pellet or suspended in culture during the 30 min immediately preceding transplant. Nonfasting blood glucose, intraperitoneal glucose tolerance test, graft histology, and graft insulin content were all used to monitor graft function up to four weeks posttransplant. Results. Maintaining islets in a compact pellet for 30 min prior to transplantation significantly reduces the proportion of transplant recipients that achieve normoglycemia (from 100% to 38%, P=0.026) and increases the proportion of apoptotic beta-cells. Conclusion. Our findings confirm that damage induced by sustained islet compaction results in poor graft outcome in mice. These findings raise concerns relating to potential damage to human islets prior to clinical transplantation, and this will be explored in further studies. |
| Databáze: | OpenAIRE |
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