Type XVI Collagen is Expressed in Factor XIIIa+ Monocyte-Derived Dermal Dendrocytes and Constitutes a Potential Substrate for Factor XIIIa
| Autor: | Shingo Tajima, Shizuko Kobayashi, Makoto Takehana, Noriko Yamaguchi, Yuko Matsubara, Akira Ishibashi, Atsushi Akagi |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male factor XIIIa Pathology medicine.medical_specialty Dystonin CD14 Lipopolysaccharide Receptors Connective tissue Nerve Tissue Proteins Dermatology dendrocyte Immunofluorescence Autoantigens Biochemistry Monocytes Dermis medicine Humans Tissue Distribution collagen XVI Molecular Biology Skin medicine.diagnostic_test Chemistry Monocyte Cell Differentiation Dendritic Cells Cell Biology Dendritic cell Middle Aged Non-Fibrillar Collagens Molecular biology Recombinant Proteins Protein Structure Tertiary Cytoskeletal Proteins medicine.anatomical_structure Cell culture monocyte Female Collagen Factor XIIIa Carrier Proteins cross-linking |
| Zdroj: | Journal of Investigative Dermatology. 118:267-274 |
| ISSN: | 0022-202X |
| DOI: | 10.1046/j.0022-202x.2001.01666.x |
| Popis: | We have previously reported that connective tissue cells in the superficial dermis preferentially express α 1 (XVI) collagen rather than those in the lower dermis. Double immunofluorescence labeling using the antibodies for α 1 (XVI) collagen and factor XIIIa (plasma transglutaminase), which is a marker of dermal dendrocytes, demonstrated that both antibodies reacted with the same cells in the superficial dermis of normal skin as well as the lesional skins of dermal dendrocyte-related disorders, dermatofibroma, and psoriasis. Dermal dendrocytes are considered to be established by a culture of peripheral blood monocytes in the presence of granulocyte macrophage-colony stimulating factor and interleukin-4. Reverse transcription–polymerase chain reaction, metabolic labeling, and immunofluorescence studies demonstrated that treatment of CD14 + peripheral blood monocytes with granulocyte macrophage-colony stimulating factor/interleukin-4 over a period of 8 d resulted in the induction of α 1 (XVI) collagen as well as factor XIIIa. The physiologic significance of colocalization of α 1 (XVI) collagen and factor XIIIa in the tissue and their coordinate induction in CD14 + monocyte-derived dendritic cells in vitro was studied. Considerable incorporation of [ 3 H]putrescine by factor XIIIa into recombinant noncollagenous domain (NC) 11 but not into collagenous domain (COL) 1·NC1 domain of the α 1 (XVI) polypeptide was found. Incubation of recombinant NC11 of α 1 (XVI) polypeptide with factor XIIIa in vitro produced a covalent cross-linking complex on sodium dodecylsulfate–polyacrylamide gel electrophoresis. The results indicate that α 1 (XVI) collagen is constitutively expressed by most dermal dendrocytes in the skin and dendritic cells differentiated from peripheral blood monocytes in vitro . Type XVI collagen is expressed in factor XIIIa + dermal dendrocytes and may form an intermolecular cross-linking through NC11 domain by the reaction catalyzed by factor XIIIa contributing to the structural integrity of factor XIIIa + dendritic cell-rich tissues. |
| Databáze: | OpenAIRE |
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