Inflammatory Profile in Response to Uncontrolled Hemorrhage in a Non-Human Primate (Rhesus Macaque) Model
| Autor: | James K. Aden, Antoni R. Macko, Forest R. Sheppard, Alexander J. Burdette, Ruth Madelaine Paredes, Randy F. Crossland |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Resuscitation Chemokine medicine.medical_treatment Gene Expression Inflammation Hemorrhage Critical Care and Intensive Care Medicine Systemic inflammation 03 medical and health sciences Medicine Animals Analysis of Variance biology business.industry Albumin biology.organism_classification Macaca mulatta Rhesus macaque Disease Models Animal 030104 developmental biology Cytokine Immunology Emergency Medicine biology.protein Cytokines Analysis of variance medicine.symptom Chemokines business |
| Zdroj: | Shock (Augusta, Ga.). 46(3 Suppl 1) |
| ISSN: | 1540-0514 |
| Popis: | BACKGROUND Uncontrolled hemorrhage (UH), the leading cause of potentially survivable combat-related death, elicits a deleterious inflammatory response. Our group previously reported an increased secretion of pro-inflammatory cytokines in a novel non-human primate model of UH; however, to better understand the molecular profile of the inflammatory response to UH, we performed a comprehensive evaluation of inflammation at the proteomic and transcriptomic level. METHODS Anesthetized rhesus macaques (n = 8) underwent UH by 60% left lobe hepatectomy T = 0 min. At T = 5 min, animals received 11 mL of 5% albumin followed by normal saline infusion to a total resuscitation volume of 20 mL/kg by T = 120 min. Blood (T = 0, 5, 20, 120, 480 min) was collected for qPCR and multiplex cytokine quantification. Results from each non-human primate (NHP) per time-point are shown. Statistical analysis by one-way ANOVA with repeated measures, P |
| Databáze: | OpenAIRE |
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