Discovery and characterization of Sip1A: a novel secreted protein from Bacillus thuringiensis with activity against coleopteran larvae
| Autor: | Chi David J, Donovan Judith C, William P. Clinton, Karina Krasomil-Osterfeld, Matthew R. Walters, James K. Roberts, Leigh H. English, James A. Baum, John W. Pitkin, Greg J. Bunkers, William P. Donovan, Oliver Ilagan, Gregory R. Heck, James T. Engleman |
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| Rok vydání: | 2006 |
| Předmět: |
Insecticides
Bacillaceae biology Bacterial Toxins Colorado potato beetle Bacillus thuringiensis General Medicine biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Bacillales Microbiology Coleoptera Western corn rootworm Bacterial Proteins Larva Botany medicine Animals Bioassay Pest Control Biological Escherichia coli Biotechnology Diabrotica undecimpunctata |
| Zdroj: | Applied Microbiology and Biotechnology. 72:713-719 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-006-0332-7 |
| Popis: | Bioassay screening of Bacillus thuringiensis culture supernatants identified strain EG2158 as having larvicidal activity against Colorado potato beetle (Leptinotarsa decemlineata) larvae. Ion-exchange fractionation of the EG2158 culture supernatant resulted in the identification of a protein designated Sip1A (secreted insecticidal protein) of approximately 38 kDa having activity against Colorado potato beetle (CPB). An oligonucleotide probe based on the N-terminal sequence of the purified Sip1A protein was used to isolate the sip1A gene. The sequence of the Sip1A protein, as deduced from the sequence of the cloned sip1A gene, contained 367 residues (41,492 Da). Recombinant B. thuringiensis and Escherichia coli harboring cloned sip1A produced Sip1A protein which had insecticidal activity against larvae of CPB, southern corn rootworm (Diabrotica undecimpunctata howardi), and western corn rootworm (Diabrotica virgifera virgifera). |
| Databáze: | OpenAIRE |
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