High-throughput screening for agonists of ROS production in live human vascular endothelial cells
| Autor: | Tomoya, Sasahara, Minako, Hoshi |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Microscopy
Science (General) General Immunology and Microbiology General Neuroscience Applied Mathematics General Mathematics Endothelial Cells Cell Biology High Throughput Screening General Biochemistry Genetics and Molecular Biology High-Throughput Screening Assays Mitochondria Q1-390 Molecular/Chemical Probes Protocol Humans Cell-based Assays Reactive Oxygen Species Fluorescent Dyes |
| Zdroj: | STAR Protocols, Vol 3, Iss 1, Pp 101053-(2022) STAR Protocols |
| ISSN: | 2435-4953 |
| Popis: | Summary Reactive oxygen species (ROS) are important physiological molecules, and identifying agonists for ROS production can yield useful tools for future research. Here we present an optimized protocol for high-throughput screening for agonists that induce ROS production. We describe the use of a fluorescent probe in human vascular endothelial cells, which can establish whether ROS production occurs in mitochondria or in the plasma membrane of live cells. For complete details on the use and execution of this profile, please refer to Sasahara et al. (2021). Graphical abstract Highlights • This is a high-throughput screening protocol for agonists that induce ROS production • ROS production in human endothelial cells is identified by fluorescence imaging • This reveals whether ROS production occurs in mitochondria or plasma membrane Reactive oxygen species (ROS) are important physiological molecules, and identifying agonists for ROS production can yield useful tools for future research. Here we present an optimized protocol for high-throughput screening for agonists that induce ROS production. We describe the use of a fluorescent probe in human vascular endothelial cells, which can establish whether ROS production occurs in mitochondria or in the plasma membrane of live cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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