Partially Reversible Adsorption of Annexin A1 on POPC/POPS Bilayers Investigated by QCM Measurements, SFM, and DMC Simulations
| Autor: | Katja Kastl, Volker Gerke, Claudia Steinem, Eike Lüthgens, Manuela Menke, Andreas Janshoff, Simon Faiß |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Time Factors
Surface Properties Lipid Bilayers Kinetics Analytical chemistry Biosensing Techniques Phosphatidylserines Microscopy Atomic Force Biochemistry chemistry.chemical_compound Adsorption Phase (matter) Microscopy Computer Simulation Particle Size Lipid bilayer Molecular Biology POPC Annexin A1 Chemistry Organic Chemistry technology industry and agriculture Membranes Artificial Quartz crystal microbalance Membrane Microscopy Fluorescence Phosphatidylcholines Molecular Medicine lipids (amino acids peptides and proteins) Stress Mechanical Monte Carlo Method |
| Zdroj: | ChemBioChem. 7:106-115 |
| ISSN: | 1439-4227 |
| DOI: | 10.1002/cbic.200500265 |
| Popis: | The kinetics of annexin A1 binding to solid-supported lipid bilayers consisting of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine (POPS; 4:1) has been investigated as a function of the calcium ion concentration in the bulk phase. Quartz crystal microbalance measurements in conjunction with scanning force microscopy, fluorescence microscopy, and computer simulations indicate that at a given Ca2+ concentration annexin A1 adsorbs irreversibly on membrane domains enriched in POPS. By contrast, annexin A1 adsorbs reversibly on the POPC-enriched phase, which is composed of single POPS molecules embedded within a POPC matrix. The overall area occupied by the POPS-enriched phase is controlled by the CaCl2 concentration. Monte Carlo simulations suggest that the area of the POPS-enriched phase increases by a factor of 7 when the Ca2+ concentration is changed from 0.01 to 1 mM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text