Clinicopathological significance of ubiquitin-specific protease 2a (USP2a), fatty acid synthase (FASN), and ErbB2 expression in oral squamous cell carcinomas
Autor: | Luiz Paulo Kowalski, Inês Nobuko Nishimoto, Dirce Maria Carraro, Fernando Augusto Soares, Sabrina Daniela da Silva, Isabela Werneck da Cunha, Edgard Graner |
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Rok vydání: | 2009 |
Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty Receptor ErbB-2 Cell Gene Expression Biology Endopeptidases Gene expression Carcinoma medicine Humans RNA Messenger Receptor Aged Aged 80 and over Mouth neoplasm Reverse Transcriptase Polymerase Chain Reaction Middle Aged Prognosis medicine.disease Neoplasm Proteins Fatty acid synthase Ki-67 Antigen medicine.anatomical_structure Oncology Tumor progression Carcinoma Squamous Cell Cancer research biology.protein Immunohistochemistry Female Mouth Neoplasms Ubiquitin-Specific Proteases Fatty Acid Synthases Oral Surgery Brazil |
Zdroj: | Oral Oncology. 45:e134-e139 |
ISSN: | 1368-8375 |
Popis: | Overexpression of fatty acid synthase (FASN) and ErbB2 has been described in oral squamous cell carcinomas (OSCC). FASN is the key lipogenic enzyme responsible for the endogenous synthesis of fatty acids and its expression can be regulated by ErbB2. The deubiquitinating enzyme (DUB) ubiquitin-specific protease 2a (USP2a) plays a critical role in prostate cancer cell survival by stabilizing the FASN protein. This study investigates whether the gene expression and the immunohistochemical status of FASN, ErbB2, and USP2a correlate with the clinicopathological characteristics of OSCC cases. A strong positive correlation among ErbB2, FASN, and USP2a expression (p=0.001) was observed by qRT-PCR in laser capture microdissected OSCC samples. Perineural infiltration was associated with ErbB2 mRNA expression (p=0.046). The presence of metastatic cervical lymph nodes was associated with FASN (p=0.002), ErbB2 (p=0.001), and USP2a (p=0.006) mRNA levels. ErbB2 staining at the cell membranes was stronger in well-differentiated lesions while a cytoplasmic positivity was found in poorly differentiated tumors. Most of the OSCC (97.06%) that showed a high positivity for FASN were also labeled for ErbB2 at the cell membranes (p=0.001). FASN and ErbB2 positivity was associated with tumor thickness and lymphatic embolization (p=0.006 and p=0.035, p=0.006 and p=0.024 respectively). The membrane expression of ErbB2 as well as FASN and Ki-67 staining were significantly associated with a high risk of recurrence by predicting both disease free survival (log-rank test, p=0.0056, p=0.0011, and p=0.0004, respectively) and overall survival (log-rank test, p=0.0005, p=0.0062, and p=0.0001, respectively). Taken together, the results presented here suggest a molecular connection among FASN, ErbB2, and USP2a in OSCC since their mRNA and protein levels were associated with tumor progression and poor prognosis. |
Databáze: | OpenAIRE |
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