Tumor necrosis factor alpha mediates orofacial discomfort in an occlusal dental interference model in rats: The role of trigeminal ganglion inflammation
| Autor: | Ramille Araújo Lima, José Vitor Mota Lemos, Joyce Ohana de Lima Martins, Raimundo Antonio de Lima Praxedes Neto, Ana Paula Negreiros Nunes Alves, Anna Clara Aragão Matos Carlos, Paulo Goberlânio de Barros Silva, Larissa Carvalho Machado |
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| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
medicine.medical_specialty Inflammation Pathology and Forensic Medicine Proinflammatory cytokine 03 medical and health sciences Trigeminal ganglion Basal (phylogenetics) 0302 clinical medicine Internal medicine Animals Medicine Rats Wistar Glial fibrillary acidic protein biology Tumor Necrosis Factor-alpha business.industry Dental occlusion 030206 dentistry Rats Endocrinology Trigeminal Ganglion Otorhinolaryngology 030220 oncology & carcinogenesis biology.protein Cytokines Periodontics Female Tumor necrosis factor alpha Oral Surgery medicine.symptom business Immunostaining |
| Zdroj: | Journal of Oral Pathology & Medicine. 49:169-176 |
| ISSN: | 1600-0714 0904-2512 |
| DOI: | 10.1111/jop.12984 |
| Popis: | BACKGROUND Tumor necrosis factor alpha (TNF-α) is a proinflammatory cytokine that plays an important role in the early stages of inflammation. In this study, we investigated its role in orofacial discomfort in rats subjected to occlusal dental interference (ODI). METHODS Female Wistar rats (180-200 g) were divided in three groups (n = 30/group): sham group, without ODI, and two experimental groups with ODI pre-treated with 0.1 mL/kg saline (ODI + SAL) or 5 mg/kg infliximab (ODI + INF) and treated every 3 days. The animals were euthanized after 1, 3, and 7 days. The number of bites and scratches and grimace scale scores were determined daily, and the bilateral trigeminal ganglion was histomorphometrically (neuronal body area) analyzed and submitted for immunohistochemistry for TNF-α, nitric oxide synthesis (NOS) neuronal (nNOS) and inducible (iNOS), peroxisome proliferator-activated receptors (PPAR) y (PPARy) and δ/β (PPARδ/β), and glial fibrillary acidic protein (GFAP). One-way/two-way ANOVA/Bonferroni tests were used (P |
| Databáze: | OpenAIRE |
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