Sequence structure character of IgNAR Sec in whitespotted bamboo shark (Chiloscyllium plagiosum)
| Autor: | Guiqian Chen, Yixin Wu, Zhengbing Lv, Lanyi Qin, Ling Wei, Rong Xu, Salma Nassor Juma, Lili Liu, Xuan Cui, Wenjie Zhang, Xin-yi Cai, Xiaofeng Jiang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Fish Proteins
0301 basic medicine Bamboo Cartilaginous fish Immunoglobulins Potential candidate Aquatic Science Biology 03 medical and health sciences Animals Environmental Chemistry Amino Acid Sequence Phylogeny Alternative splicing Disulfide bond 04 agricultural and veterinary sciences General Medicine biology.organism_classification Receptors Antigen 030104 developmental biology Gene Expression Regulation Evolutionary biology Sharks 040102 fisheries 0401 agriculture forestry and fisheries Chiloscyllium plagiosum Hinge region Sequence structure |
| Zdroj: | Fish & Shellfish Immunology. 102:140-144 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2020.04.037 |
| Popis: | Whitespotted bamboo shark (Chiloscyllium plagiosum) is a demersal cartilaginous fish with an adaptive immune system founded upon immunoglobulins. In this manuscript, we characterize the IgNAR of the whitespotted bamboo shark. A newly discovered alternative splicing form of IgNAR Sec (IgNARshort (ΔC2-C3) Sec) was identified, in which the C1 domain was spliced directly to the C4 domain, the process resulted in a molecule containing three constant domains. However, a single unpaired cysteine remains in the highly flexible hinge region, contributing in the formation of an interchain disulfide bond. Two types of C1 domain were found, and the one lacking a short α-helix showed lower proportion. This finding suggests that short α-helices might be important to the stability of IgNAR. High-throughput sequencing revealed that the percentage of VNAR types significantly vary between the diverse species of sharks. The variable region of IgNAR (the VNAR) with small size and stabilization is a potential candidate for immunotherapeutic agents. The structure and stability analysis in this manuscript may be useful in future biomedical applications. |
| Databáze: | OpenAIRE |
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