Insights into GATA-1-Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis
| Autor: | Laura Riva, Ernest Fraenkel, Yong Cheng, Huafeng Xie, Mitchell J. Weiss, Stuart H. Orkin, Alan B. Cantor, Bradley E. Bernstein, Duonan Yu, Yocheved Schindler, Tyler B. Moran, Ming Yu, Ross C. Hardison |
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| Rok vydání: | 2009 |
| Předmět: |
Transcriptional Activation
Chromatin Immunoprecipitation Molecular Sequence Data Biotin Polycomb-Group Proteins Repressor Regulatory Sequences Nucleic Acid Biology Article Mice Cell Line Tumor Polycomb-group proteins Animals Gene silencing Biotinylation GATA1 Transcription Factor Gene Silencing Transcription factor Gene Molecular Biology Genetics Regulation of gene expression Binding Sites Genome Base Sequence Models Genetic Gene Expression Regulation Leukemic Gene Expression Profiling Computational Biology Sequence Analysis DNA Cell Biology Chromatin Repressor Proteins Gene expression profiling embryonic structures Streptavidin Protein Binding TAL1 |
| Zdroj: | Molecular Cell. 36(4):682-695 |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2009.11.002 |
| Popis: | The transcription factor GATA-1 is required for terminal erythroid maturation and functions as an activator or repressor depending on gene context. Yet its in vivo site selectivity and ability to distinguish between activated versus repressed genes remain incompletely understood. In this study, we performed GATA-1 ChIP-seq in erythroid cells and compared it to GATA-1 induced gene expression changes. Bound and differentially expressed genes contain a greater number of GATA binding motifs, a higher frequency of palindromic GATA sites, and closer occupancy to the transcriptional start site versus non-differentially expressed genes. Moreover, we show that the transcription factor Zbtb7a occupies GATA-1 bound regions of some direct GATA-1 target genes, that the presence of SCL/TAL1 helps distinguish transcriptional activation versus repression, and that Polycomb Repressive Complex 2 (PRC2) is involved in epigenetic silencing of a subset of GATA-1 repressed genes. These data provide insights into GATA-1 mediated gene regulation in vivo. |
| Databáze: | OpenAIRE |
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