Postsynthetic Domain Assembly with NpuDnaE and SspDnaB Split Inteins
| Autor: | Naiyi Li, Sheldon Park, Daniel Demonte |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Genetics
Protein subunit Protein domain Bioengineering Sequence (biology) General Medicine Computational biology Biology Applied Microbiology and Biotechnology Biochemistry Inteins Protein Structure Tertiary Protein splicing RNA splicing Escherichia coli Protein Splicing Peptide bond Intein DnaB Helicases Molecular Biology dnaB helicase DNA Polymerase III Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 177:1137-1151 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-015-1802-0 |
| Popis: | Inteins are protein segments embedded in frame within a precursor sequence that catalyze a self-excision reaction and ligate the flanking sequences with a standard peptide bond. Split inteins are expressed as two separate polypeptide fragments and trans-splice upon subunit association. Split inteins have found use in biotechnology applications but their use in postsynthetic domain assembly in vivo has been limited to the ligation of two protein domains. Alternatively, they have been used to splice three domains and fragments in vitro. To further develop split intein-based applications in vivo, we have designed a cell-based assay for the postsynthetic splicing of three protein domains using orthogonal split inteins. Using naturally and artificially split inteins, NpuDnaE and SspDnaB, we show that a multidomain protein of 128 kDa can be assembled in Escherichia coli from individually expressed domains. In the current system, the main bottleneck in achieving high yield of tandem trans-spliced product appears to be the limited solubility of the SspDnaB precursors. Optimizing protein solubility should be important to achieve efficient combinatorial synthesis of protein domains in the cell. |
| Databáze: | OpenAIRE |
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