| Autor: |
Hideki Takakura, Mano Horinaka, Ayaka Imai, Yuichi Aono, Toshimasa Nakao, Shingo Miyamoto, Yosuke Iizumi, Motoki Watanabe, Takumi Narita, Hideki Ishikawa, Michihiro Mutoh, Toshiyuki Sakai |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Journal of clinical biochemistry and nutrition. 70(2) |
| ISSN: |
0912-0009 |
| Popis: |
As colon cancer is one of the most common cancers in the world, practical prevention strategies for colon cancer are needed. Recently, treatment with aspirin and/or 5-aminosalicylic acid-related agents was reported to reduce the number of intestinal polyps in patients with familial adenomatous polyposis. To evaluate the mechanism of aspirin and 5-aminosalicylic acid for suppressing the colon polyp growth, single and combined effects of 5-aminosalicylic acid and sodium salicylate (metabolite of aspirin) were tested in the two human colon cancer cells with different cyclooxygenase-2 expression levels and intestinal polyp-derived cells from familial adenomatous polyposis model mouse. The combination induced cell-cycle arrest at the G1 phase along with inhibition of cell growth and colony-forming ability in these cells. The combination reduced cyclin D1 via proteasomal degradation and activated retinoblastoma protein. The combination inhibited the colony-forming ability of mouse colonic mucosa cells by about 50% and the colony-forming ability of mouse intestinal polyp-derived cells by about 90%. The expression level of cyclin D1 in colon mucosa cells was lower than that in intestinal polyp-derived cells. These results suggest that this combination may be more effective in inhibiting cell growth of intestinal polyps through cyclin D1 down-regulation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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