Effect of berberine on LPS-induced expression of NF-κB/MAPK signalling pathway and related inflammatory cytokines in porcine intestinal epithelial cells

Autor: Huang Jing, Xu Yuwei, Jin Meilan, Xiong Wen, Wang Zi-li, Li Xueying, Zhang Zhu, Li Chunlin, Zeng Rongrong
Rok vydání: 2020
Předmět:
Lipopolysaccharides
lcsh:Immunologic diseases. Allergy
0301 basic medicine
Berberine
Swine
Immunology
Anti-Inflammatory Agents
Pharmacology
Microbiology
NF-κB
Proinflammatory cytokine
03 medical and health sciences
chemistry.chemical_compound
MAPKs
0302 clinical medicine
Phellodendron
Escherichia coli
Animals
Phellodendron chinense
Intestinal Mucosa
Medicinal plants
Molecular Biology
Cells
Cultured
Escherichia coli Infections
Mitogen-Activated Protein Kinase Kinases
biology
Alkaloid
MAPK signalling
NF-kappa B
Original Articles
Cell Biology
Coptis chinensis
biology.organism_classification
030104 developmental biology
Infectious Diseases
Gene Expression Regulation
chemistry
030220 oncology & carcinogenesis
porcine intestinal epithelial cells inflammatory factors
Cytokines
Inflammation Mediators
lcsh:RC581-607
Coptis
Signal Transduction
Zdroj: Innate Immunity, Vol 26 (2020)
Innate Immunity
ISSN: 1753-4267
1753-4259
DOI: 10.1177/1753425920930074
Popis: Berberine is an alkaloid extracted from medicinal plants such as Coptis chinensis and Phellodendron chinense. It possesses anti-inflammatory, anti-tumour and anti-oxidation properties, and regulates Glc and lipid metabolism. This study explored the mechanisms of the protective effects of berberine on barrier function and inflammatory damage in porcine intestinal epithelial cells (IPEC-J2) induced by LPS. We first evaluated the effects of berberine and LPS on cell viability. IPEC-J2 cells were treated with 5 μg/ml LPS for 1 h to establish an inflammatory model, and 75, 150 and 250 μg/ml berberine were used in further experiments. The expression of IL-1β, IL-6 and TNF-α was measured by RT-PCR. The key proteins of the NF-κB/MAPK signalling pathway (IκBα, p-IκBα, p65, p-p65, c-Jun N-terminal kinase (JNK), p-JNK, p38, p-p38, ERK1/2 and p-ERK1/2) were detected by Western blot. Upon exposure to LPS, IL-1β, IL-6 and TNF-α mRNA levels and p-IκBα p-p65 protein levels were significantly enhanced. Pre-treatment with berberine reduced the expression of inflammatory factors and was positively correlated with its concentration, and dose dependently inhibited the expression of IκBα, p-IκBα, p-p65, p-p38 and JNK. These results demonstrated that pre-treating intestinal epithelial cells with berberine was useful in preventing and treating diarrhoea induced by Escherichia coli in weaned pigs.
Databáze: OpenAIRE
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