Correlation between inhibin secretion and damage of seminiferous tubules in a model of experimental autoimmune orchitis
| Autor: | M O, Suescun, L, Lustig, R S, Calandra, N P, Groome, S, Campo |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Germinal epithelium damage of seminiferous tubules endocrine system medicine.medical_specialty Endocrinology Diabetes and Metabolism Orchitis Biology Active immunization Peripheral blood mononuclear cell Autoimmune Diseases Rats Sprague-Dawley Endocrinology Internal medicine Testis medicine Animals Inhibins Ciencias Exactas Analysis of Variance Sertoli Cells Inhibin secretion Organ Size Luteinizing Hormone Seminiferous Tubules Sertoli cell medicine.disease Immunohistochemistry Rats rats medicine.anatomical_structure Seminiferous tubule Models Animal inhibin secretion Follicle Stimulating Hormone |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| ISSN: | 1479-6805 0022-0795 |
| DOI: | 10.1677/joe.0.1700113 |
| Popis: | The aim of the present study was to evaluate inhibin secretion in rats with autoimmune orchitis. As we have previously described, experimental autoimmune orchitis (EAO) induced in rats by active immunization with testis homogenate and adjuvants is characterized by an interstitial mononuclear cell infiltrate and sloughing of the germinal epithelium. At 120 days after the first immunization 60% of the rats exhibited a severe orchitis with large areas of aspermatogenic seminiferous tubules in which only spermatogonia and Sertoli cells with cytoplasmic vacuolization remained attached to the tubular wall. None of the untreated (N) or control (C) rats revealed pathological alterations. Sixty percent decrease in testis weight was observed in rats with EAO compared with N or C groups. A 3-fold increase in serum FSH levels was observed in rats with EAO compared with N or C groups (19·8 ± 3·7 vs 5·6 ± 0·3 and 5·9 ± 0·1 ng/ml respectively). A significant decrease in inhibin B levels was observed in rats with EAO when compared with N or C groups (40 ± 4·6 vs 207 ± 38·8 and 221·4 ± 28·6 pg/ml respectively). An inverse correlation between inhibin B and FSH serum levels and a direct correlation between inhibin B and testis weight were found. Strong expression of the inhibin α-subunit in Sertoli cells of untreated and control rats was observed; this subunit was undetectable or poorly detectable in rats with orchitis. Positive staining for the inhibin α-subunit was also observed in Leydig cells of all groups studied. In conclusion, using a model of autoimmune orchitis our results show that circulating inhibin B levels and inhibin α-subunit expression in Sertoli cell cytoplasm closely correlate with the degree of damage of the germinal epithelium. Instituto Multidisciplinario de Biología Celular Facultad de Ciencias Exactas |
| Databáze: | OpenAIRE |
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