fMLP causes degranulation followed by regranulation in rat nasal glands
| Autor: | Iris F. Ueki, Jay A. Nadel, Takashi Nakanaga, Seon-Tae Kim |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Neutrophils Receptor ErbB-2 Neutrophile Cell Degranulation Internal medicine Animals Medicine Submucosal glands Inhalation business.industry Mucin Degranulation Nasal glands Immunohistochemistry Mucus Rats Inbred F344 Rats ErbB Receptors N-Formylmethionine Leucyl-Phenylalanine Nasal Mucosa Endocrinology Otorhinolaryngology Granulation Tissue Nasal administration business |
| Zdroj: | The Laryngoscope. 113:1998-2003 |
| ISSN: | 0023-852X |
| Popis: | OBJECTIVE To determine the mechanism of mucus production by nasal glands. STUDY DESIGN Because neutrophilic inflammation is associated with mucus hypersecretion in disease states, here we examine the role of neutrophil recruitment in mucous cell degranulation and regranulation in rat nasal glands. METHODS N-formyl-methionyl-leucyl-phenylalanine (fMLP) was aerosolized intranasally in rats (n = 5), and its effects on degranulation and regranulation of submucosal glands were evaluated by Alcian blue/periodic acid-Schiff (AB/PAS) staining and by immunolocalization of neutrophils and epidermal growth factor receptor (EGF-R). RESULTS In control subjects, glands were filled with mucin. After fMLP inhalation, degranulation, 31.7 +/- 0.8% (P |
| Databáze: | OpenAIRE |
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