Lipid phase separation induced by the apolar polyisoprenoid squalane demonstrates its role in membrane domain formation in archaeal membranes
| Autor: | Bruno Demé, Marta Salvador-Castell, Phil M. Oger, Judith Peters |
|---|---|
| Přispěvatelé: | Microbiologie, adaptation et pathogénie (MAP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Microbiology of Extreme Environments (M2E), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut Laue-Langevin (ILL), ILL, Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Squalene
Lipid Bilayers 02 engineering and technology [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] 010402 general chemistry 01 natural sciences Domain formation chemistry.chemical_compound neutron diffraction Squalane Electrochemistry Molecule General Materials Science Spectroscopy phospholipids Phosphocholine biology [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] Chemistry Bilayer Surfaces and Interfaces 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification Archaea 0104 chemical sciences [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biophysics Thermococcus barophilus Membrane membrane domains Biophysics squalane lipid phase separation lipids (amino acids peptides and proteins) 0210 nano-technology [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft] |
| Zdroj: | Langmuir Langmuir, American Chemical Society, In press, ⟨10.1021/acs.langmuir.0c00901⟩ |
| ISSN: | 0743-7463 1520-5827 |
| DOI: | 10.1021/acs.langmuir.0c00901⟩ |
| Popis: | International audience; Archaea synthesize methyl-branched, ether phospholipids, which confer the archaeal membrane exceptional physico-chemical properties. A novel membrane organization has been proposed recently to explain the thermal and high pressure tolerance of the polyextremophilic archaeon Thermococcus barophilus. According to this theoretical model, apolar molecules could populate the midplane of the bilayer and could alter the physico-chemical properties of the membrane, among which the possibility to form membrane domains. We tested this hypothesis using neutron diffraction on a model archaeal membrane composed of two archaeal diether lipids with phosphocholine and phosphoethanolamine headgroups in presence of the apolar polyisoprenoid squalane. We show that squalane is inserted in the midplane at a maximal concentration between 5 and 10 mol% and that squalane can modify the lateral organization of the membrane and induces the coexistence of separate phases. The lateral reorganization is temperature- and squalane concentration-dependent and could be due to the release of lipid chain frustration and the induction of a negative curvature in the lipids. |
| Databáze: | OpenAIRE |
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