The effects of S(-)-, R(+)-, and racemic bupivacaine on lysophosphatidate-induced priming of human neutrophils
| Autor: | Markus W. Hollmann, Klaus Hahnenkamp, Marcel E. Durieux, Katrin S. Kurz, Danja Struemper, Christel G. den Bakker, Noud S. Berkelmans, Susanne Herroeder |
|---|---|
| Přispěvatelé: | Anesthesiology |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
medicine.drug_class
Neutrophils Neutrophile Voltage clamp Xenopus Priming (immunology) Cytochrome c Group Pharmacology In Vitro Techniques chemistry.chemical_compound Superoxides Lysophosphatidic acid medicine Animals Humans Anesthetics Local Bupivacaine biology Local anesthetic business.industry Stereoisomerism biology.organism_classification Oxidants In vitro Kinetics Anesthesiology and Pain Medicine Biochemistry chemistry Oocytes Lysophospholipids business Algorithms medicine.drug Signal Transduction |
| Zdroj: | Anesthesia and analgesia, 97(4), 1053-8, table of contents. Lippincott Williams and Wilkins |
| ISSN: | 0003-2999 |
| Popis: | UNLABELLED Local anesthetics modulate inflammatory responses and may therefore be potentially useful in mitigating perioperative inflammatory injury. The inflammatory modulating effects of S(-)-bupivacaine are not known. Therefore, we compared the effects of S(-)-bupivacaine, R(+)-bupivacaine, and racemic bupivacaine on neutrophil function and receptor signaling. Priming (by lysophosphatidic acid [LPA]) and activation (by N-formylmethionine-leucyl-phenylalanine) of superoxide release by isolated human neutrophils was studied by using a cytochrome c-reduction assay. LPA receptor signaling in Xenopus oocytes was studied by using voltage clamp. All three local anesthetics were without effect on activation. S(-)-Bupivacaine inhibited priming more than did racemic bupivacaine; R(+)-bupivacaine was without effect. At 10(-4) M, S(-)-bupivacaine inhibited approximately 50%. Comparable results were obtained in our recombinant model, where S(-)-bupivacaine most effectively inhibited LPA signaling. Compared with racemic bupivacaine and other anesthetics, S(-)-bupivacaine appears particularly effective in suppressing neutrophil priming, a process responsible in part for the overactive neutrophil response. IMPLICATIONS Overactive inflammatory responses underlie several perioperative disorders. Compared with racemic bupivacaine and other anesthetics, S(-)-bupivacaine appears particularly effective in suppressing neutrophil priming, a process responsible in part for the overactive neutrophil response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|