A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy

Autor: Pin Dong, Ernesto Rafael Osorio-Blanco, Amit Kumar, Gregor Nagel, Marcelo Calderón, Lydia M. Bouchet, Christian Teutloff, Alexa Patzelt, Marius Nieke, Monika Schäfer-Korting, Ulrike Alexiev, Silke B. Lohan, Johannes Stellmacher, Martina C. Meinke
Rok vydání: 2021
Předmět:
Fluorescence-lifetime imaging microscopy
Rhodamine B
spin labels
multiplexed FLIM–
skin penetration
Analytical Methods
01 natural sciences
law.invention
Spin probe
chemistry.chemical_compound
law
penetration properties
Electron paramagnetic resonance
Spin label
Research Articles
Skin
technologies
chemistry.chemical_classification
Molecular Structure
rhodamine-B
General Medicine
Fluorescence
fluorescence
500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
multiplexed FLIM–EPR spectroscopy
Research Article
EPR spectroscopy
fluorophore
Materials science
dexamethasone
Nanotechnology
in-vitro
010402 general chemistry
Catalysis
Humans
3-carboxy-proxyl (PCA)
Spectroscopy
Fluorescent Dyes
nanocarriers
Rhodamines
010405 organic chemistry
Biomolecule
Electron Spin Resonance Spectroscopy
General Chemistry
Rhodamine 
0104 chemical sciences
Microscopy
Fluorescence
chemistry
nanoparticles
barrier function
Zdroj: Addi. Archivo Digital para la Docencia y la Investigación
instname
Addi: Archivo Digital para la Docencia y la Investigación
Universidad del País Vasco
Angewandte Chemie (International Ed. in English)
ISSN: 1521-3773
1433-7851
DOI: 10.1002/anie.202012852
Popis: Simultaneous visualization and concentration quantification of molecules in biological tissue is an important though challenging goal. The advantages of fluorescence lifetime imaging microscopy (FLIM) for visualization, and electron paramagnetic resonance (EPR) spectroscopy for quantification are complementary. Their combination in a multiplexed approach promises a successful but ambitious strategy because of spin label‐mediated fluorescence quenching. Here, we solved this problem and present the molecular design of a dual label (DL) compound comprising a highly fluorescent dye together with an EPR spin probe, which also renders the fluorescence lifetime to be concentration sensitive. The DL can easily be coupled to the biomolecule of choice, enabling in vivo and in vitro applications. This novel approach paves the way for elegant studies ranging from fundamental biological investigations to preclinical drug research, as shown in proof‐of‐principle penetration experiments in human skin ex vivo.
A novel multi‐label synthetic platform for a functional dual fluorescence–spin label probe enables the non‐destructive simultaneous quantification and visualization of molecules in biological tissue. Multiplexed FLIM and EPR spectroscopy avoids analytical inconsistencies between both techniques. Beside tissue applications, molecular spectroscopic studies of biomolecular conformation, structure, dynamics, and microenvironment are feasible.
Databáze: OpenAIRE
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