Increased secretion and expression of amylin in spontaneously diabetic Goto-Kakizaki rats treated with rhGLP-1 (7-36)
| Autor: | Meng Liu, Xin Gao, Neng-Neng Cheng, Duan Li, Hong-Bo Weng, Qian Gu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Blood Glucose
Male endocrine system medicine.medical_specialty Amyloid Time Factors endocrine system diseases medicine.medical_treatment Amylin Gene Expression macromolecular substances Type 2 diabetes Goto kakizaki Eating Glucagon-Like Peptide 1 Diabetes mellitus Internal medicine medicine Animals Humans Hypoglycemic Agents Insulin Pharmacology (medical) Secretion RNA Messenger Rats Wistar Pharmacology Chemistry General Medicine Fasting medicine.disease Glucagon-like peptide-1 Immunohistochemistry Recombinant Proteins Islet Amyloid Polypeptide Rats Endocrinology Glucose Diabetes Mellitus Type 2 |
| Zdroj: | Acta pharmacologica Sinica. 29(5) |
| ISSN: | 1745-7254 |
| Popis: | To investigate the effect of recombined human glucagon-like peptide 1 (rhGLP-1 [7-36]) on the secretion and expression of amylin in Goto-Kakizaki (GK) rats.The GK rats were treated with rhGLP-1 (7-36) 56 and 133 mug/kg subcutaneously for 12 weeks. The fasting and post-prandial blood glucose levels were measured. The plasma amylin concentration was measured by ELISA. The transcription levels of amylin and insulin mRNA were evaluated by fluorescent-quantitative- PCR. Immunohistochemistry was utilized to detect the amylin protein. Histological examination was assayed by light microscopy.Treatment with rhGLP-1 (7-36) caused a significant reduction of post-prandial blood glucose levels in the GK rats (P0.05). The plasma amylin levels of the GK rats were lower than those of Wistar rats after the glucose administration (P0.01). Treatment with rhGLP-1 (7-36) exhibited a marked elevation of the glucose-stimulated plasma amylin level (P0.05) and slight histological amelioration. The amylin expression was augmented in the rhGLP-1 (7-36)-treated GK rat pancreas. Amylin and insulin mRNA were also highly expressed in the treated GK rats (P0.05). However, the ratio of amylin to insulin mRNA was significantly decreased by treatment with rhGLP-1 (7-36).RhGLP-1 (7-36) stimulates the secretion and expression of amylin, and exerts a beneficial effect on the ratio of amylin to insulin mRNA. These findings suggest that GLP-1 and GLP-1 analogs are ideal candidates for the treatment of type 2 diabetes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text