Phytochemical and antioxidant properties of different solvent extracts of Kirkia wilmsii tubers
| Autor: | Simon S. Mnyakeni-Moleele, Jane M. Misihairabgwi, Paul Eanas Lesedi Mojapelo, Kayini Chigayo |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Flavonoid Total phenol Phytochemical 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) chemistry.chemical_compound Gallic acid Phenols Kirkia wilmsii chemistry.chemical_classification Chloroform Chromatography 010405 organic chemistry fungi Extraction (chemistry) food and beverages 0104 chemical sciences Solvent chemistry Biochemistry Methanol Antioxidant 010606 plant biology & botany |
| Zdroj: | Asian Pacific Journal of Tropical Biomedicine. 6:1037-1043 |
| ISSN: | 2221-1691 |
| DOI: | 10.1016/j.apjtb.2016.10.004 |
| Popis: | ObjectiveTo determine suitable phytochemical extraction solvents, screen for phytochemicals, determine the total phenol and flavonoid contents and the antioxidant activities of different solvent extracts of Kirkia wilmsii (K. wilmsii), an ethnomedicine in South Africa.MethodsExtractions were performed from dried tubers of the K. wilmsii plant, using several solvents and varying extraction times. Extract yields were determined and suitable extraction solvents were selected. Total phenol and flavonoid contents of the extracts were determined spectrophotometrically using gallic acid and quercetin as standards. The free radical scavenging activity of the extracts was investigated using 1,1-diphenyl-2-picrylhydrazyl radical.ResultsPhytochemical screening confirmed the presence of phenolics, flavonoids, terpenoids, tannins, cardenolide deoxy sugars and reducing sugars. Of the 12 solvent extracts used, six gave yields higher than 5%, while the other six gave yields less than 1%. The highest extract yield of 52.9% was obtained using 80% methanol while the lowest yield of 7.3% was obtained using ethanol at 60 min. The 80% methanol, methanol/chloroform/water (12:5:3) and 60% methanol extracts were significantly higher than those of ethanol, methanol and water (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|