A restorer‐of‐fertility‐like pentatricopeptide repeat protein promotes cytoplasmic male sterility in Arabidopsis thaliana
| Autor: | Stéphanie Durand, Françoise Budar, Anthony Ricou, Matthieu Simon, Christine Camilleri, Noémie Dehaene |
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| Přispěvatelé: | Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, IJPB's Plant Observatory technological platforms Saclay Plant Sciences-SPS ANR-17-EUR-0007INRAE Biology and Plant Breeding department (CONTRAD grant) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Mitochondrial DNA cytoplasmic male sterility like PPR Plant Infertility Arabidopsis thaliana Sterility Quantitative Trait Loci Arabidopsis Plant Science Biology Genes Plant 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Gene expression Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology natural variation Amino Acid Sequence Allele Gene 030304 developmental biology 0303 health sciences Arabidopsis Proteins Cytoplasmic male sterility pollen development Cell Biology biology.organism_classification Rf‐ Pollen Pentatricopeptide repeat 010606 plant biology & botany |
| Zdroj: | Plant Journal Plant Journal, Wiley, 2021, 105 (1), pp.124-135. ⟨10.1111/tpj.15045⟩ |
| ISSN: | 0960-7412 1365-313X |
| Popis: | International audience; Pentatricopeptide repeat (PPR) proteins form a large family of proteins targeted to organelles, where they post-transcriptionally modulate gene expression through binding to specific RNA sequences. Among them, the mitochondria-targeted restorer-of-fertility (Rf) PPRs inhibit peculiar mitochondrial genes that are detrimental to male gametes and cause cytoplasmic male sterility (CMS). Here, we revealed three nuclear loci involved in CMS in a cross between two distant Arabidopsis thaliana strains, Sha and Cvi-0. We identified the causal gene at one of these loci as RFL24, a conserved gene encoding a PPR protein related to known Rf PPRs. By analysing fertile revertants obtained in a male sterile background, we demonstrate that RFL24 promotes pollen abortion, in contrast with the previously described Rf PPRs, which allow pollen to survive in the presence of a sterilizing cytoplasm. We show that the sterility caused by the RFL24 Cvi-0 allele results from higher expression of the gene during early pollen development. Finally, we predict a binding site for RFL24 upstream of two mitochondrial genes, the CMS gene and the important gene cob. These results suggest that the conservation of RFL24 is linked to a primary role of ensuring a proper functioning of mitochondria, and that it was subsequently diverted by the CMS gene to its benefit. |
| Databáze: | OpenAIRE |
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