Gamma/Delta T-Cells Enhance Carboplatin-induced Cytotoxicity Towards Advanced Bladder Cancer Cells
| Autor: | Der Yang Cho, Yueh Pan, Liang-Ming Lee, Ya-Hsu Chiu, Jacqueline Whang-Peng, Ping-Hsiao Shih, Chi-Hao Hsiao, Shao Chih Chiu, Yu-Ching Wen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
Cell Survival T-Lymphocytes medicine.medical_treatment Antineoplastic Agents Carboplatin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine T-Lymphocyte Subsets Cell Line Tumor Humans Medicine Viability assay Gamma delta T cell Cytotoxicity Chemotherapy Bladder cancer business.industry Receptors Antigen T-Cell gamma-delta General Medicine medicine.disease Cytolysis Zoledronic acid medicine.anatomical_structure Urinary Bladder Neoplasms Oncology chemistry 030220 oncology & carcinogenesis Cancer research business medicine.drug |
| Zdroj: | Anticancer Research. 40:5221-5227 |
| ISSN: | 1791-7530 0250-7005 |
| Popis: | BACKGROUND/AIM Bladder cancer (BLCA, urothelial bladder cancer) is one of the most common malignancies with increasing incidence and mortality worldwide. Poor diagnosis and the limitation of treatment is still an unmet need in clinical practice. γδ T-Cells have been paid increasing attention because of their potent cytotoxicity against tumors. Herein, we investigated the cytolytic effect of γδ T-cells in combination with the chemotherapeutic drug, carboplatin, against BLCA cells. MATERIALS AND METHODS The standard protocol for the induction and expansion of peripheral blood mononuclear cell-derived γδ T-cells was a zoledronic acid/interleukin-2-based medium system for 2 weeks. The cytotoxicity of γδ T-cells with and without carboplatin against BLCA cells was examined. RESULTS After incubation, T-cell receptor-positive γδ T-cells showed a natural killer cell-like phenotypic characteristic and dose-dependently increased cytotoxicity against BLCA cells. Interestingly, we found that in advanced BLCA cells, which were more resistant to carboplatin, the cell viability was significantly (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|