The Influence of an Adrenergic Antagonist Guanethidine on the Distribution Pattern and Chemical Coding of Caudal Mesenteric Ganglion Perikarya and Their Axons Supplying the Porcine Bladder
| Autor: | A. Bossowska, Urszula Mazur, Ewa Lepiarczyk, W. Markiewicz, Paweł Janikiewicz, Mariusz Majewski, Barbara Wasilewska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
pig
Adrenergic Antagonists medicine.medical_specialty Inferior mesenteric ganglion Swine QH301-705.5 Neuropeptide Dopamine beta-Hydroxylase noradrenergic nerve fibers Article Catalysis Inorganic Chemistry Nerve Fibers Internal medicine medicine Adrenergic antagonist Animals Neuropeptide Y Physical and Theoretical Chemistry Biology (General) Mesenteric ganglion Molecular Biology QD1-999 Spectroscopy Guanethidine Ganglia Sympathetic Urinary bladder Tyrosine hydroxylase Chemistry Organic Chemistry neuropeptides inferior mesenteric ganglion General Medicine Neuropeptide Y receptor Axons Computer Science Applications medicine.anatomical_structure Endocrinology guanethidine Female urinary bladder medicine.drug |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 4896, p 4896 (2021) International Journal of Molecular Sciences Volume 22 Issue 9 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | This study was aimed at disclosing the influence of intravesically instilled guanethidine (GUA) on the distribution, relative frequency and chemical coding of both the urinary bladder intramural sympathetic nerve fibers and their parent cell bodies in the caudal mesenteric ganglion (CaMG) in juvenile female pigs. GUA instillation led to a profound decrease in the number of perivascular nerve terminals. Furthermore, the chemical profile of the perivascular innervation within the treated bladder also distinctly changed, as most of axons became somatostatin-immunoreactive (SOM-IR), while in the control animals they were found to be neuropeptide Y (NPY)-positive. Intravesical treatment with GUA led not only to a significant decrease in the number of bladder-projecting tyrosine hydroxylase (TH) CaMG somata (94.3 ± 1.8% vs. 73.3 ± 1.4% control vs. GUA-treated pigs), but simultaneously resulted in the rearrangement of their co-transmitters repertoire, causing a distinct decrease in the number of TH+/NPY+ (89.6 ± 0.7% vs. 27.8 ± 0.9%) cell bodies and an increase in the number of SOM-(3.6 ± 0.4% vs. 68.7 ± 1.9%), calbindin-(CB 2.06 ± 0.2% vs. 9.1 ± 1.2%) or galanin-containing (GAL 1.6 ± 0.3% vs. 28.2 ± 1.3%) somata. The present study provides evidence that GUA significantly modifies the sympathetic innervation of the porcine urinary bladder wall, and thus may be considered a potential tool for studying the plasticity of this subdivision of the bladder innervation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|