Comparison Between the Genotoxicity ofcis-Pt(II) Complex of 3-Aminoflavone andcis-DDP in Lymphocytes Evaluated by the Comet Assay
| Autor: | Elzbieta Zyner, B. Kosmider, Justyn Ochocki, Regina Osiecka |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
DNA Repair
Organoplatinum Compounds Cell Survival Stereochemistry DNA repair DNA damage Health Toxicology and Mutagenesis Biology Toxicology medicine.disease_cause chemistry.chemical_compound medicine Humans Lymphocytes Cytotoxicity Incubation Cells Cultured Pharmacology Chemical Health and Safety Public Health Environmental and Occupational Health General Medicine Molecular biology DNA Repair Kinetics Comet assay chemistry Female Comet Assay Cisplatin DNA Genotoxicity DNA Damage Mutagens |
| Zdroj: | Drug and Chemical Toxicology. 28:231-244 |
| ISSN: | 1525-6014 0148-0545 |
| Popis: | cis-bis(3-aminoflavone)dichloroplatinum(II) [cis-Pt(II) complex of 3-aminoflavone] is an analog of cis-DDP characterized by low cytotoxicity and anticancer properties in vivo. In order to evaluate genotoxic properties of this chemical compound, the comet assay in human lymphocytes was used. The analysis of DNA damage after 1-h cell incubation with cis-Pt(II) complex of 3-aminoflavone and cis-DDP was carried out, and DNA repair kinetics were evaluated after 0.5-h, 1-h, and 1.5-h postexposure incubation. It has been shown that cis-Pt(II) complex of 3-aminoflavone causes the increase in tail moments in comparison with cis-DDP. On the other hand, the decrease in these values caused by cis-DDP was connected mainly with the presence of DNA and DNA-protein crosslinks. The decrease in tail moments after cis-Pt(II) complex of 3-aminoflavone lymphocyte treatment was already observed after 0.5-h postexposure incubation, whereas in the variant with hydrogen peroxide application these values after cis-Pt(II) complex of 3-aminoflavone addition were higher in comparison with cis-DDP. Results obtained on the basis of the comet assay could confirm the occurrence of DNA breaks and cross-links induced by cis-Pt(II) complex of 3-aminoflavone. |
| Databáze: | OpenAIRE |
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