The piggyBac-derived protein 5 (PGBD5) transposes both the closely and the distantly related piggyBac-like elements Tcr-pble and Ifp2
| Autor: | Linda Beauclair, Alex Kentsis, Louis Tsakou-Ngouafo, Hugues Dardente, Yves Bigot, Pierre Pontarotti, Laura Helou, Benoît Piégu, Thierry Lecomte |
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| Přispěvatelé: | Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Groupe innovation et ciblage cellulaire (GICC), EA 7501 [2018-...] (GICC EA 7501), Université de Tours (UT), Memorial Sloane Kettering Cancer Center [New York], Weill Medical College of Cornell University [New York], Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Tours |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Transposable element
[SDV]Life Sciences [q-bio] PGBD5 Gene Transposases Biology Article Transposition (music) Mice 03 medical and health sciences chemistry.chemical_compound domestication 0302 clinical medicine Structural Biology Molecular evolution transposition Animals Humans DNA binding Molecular Biology Gene Phylogeny Transposase 030304 developmental biology Genetics 0303 health sciences molecular evolution Palindrome transposable element chemistry DNA Transposable Elements 030217 neurology & neurosurgery DNA Transcription Factors |
| Zdroj: | Journal of Molecular Biology Journal of Molecular Biology, 2021, 433 (7), pp.1-16. ⟨10.1016/j.jmb.2021.166839⟩ Journal of Molecular Biology, Elsevier, 2021, 433 (7), pp.1-16. ⟨10.1016/j.jmb.2021.166839⟩ J Mol Biol |
| ISSN: | 0022-2836 1089-8638 |
| DOI: | 10.1016/j.jmb.2021.166839⟩ |
| Popis: | International audience; The vertebrate piggyBac derived transposase 5 (PGBD5) encodes a domesticated transposase, which is active and able to transpose its distantly related piggyBac-like element (pble), Ifp2. This raised the question whether PGBD5 would be more effective at mobilizing a phylogenetically closely related pble element. We aimed to identify the pble most closely related to the pgbd5 gene. We updated the landscape of vertebrate pgbd genes to develop efficient filters and identify the most closely related pble to each of these genes. We found that Tcr-pble is phylogenetically the closest pble to the pgbd5 gene. Furthermore, we evaluated the capacity of two murine and human PGBD5 isoforms, Mm523 and Hs524, to transpose both Tcr-pble and Ifp2 elements. We found that both pbles could be transposed by Mm523 with similar efficiency. However, integrations of both pbles occurred through both proper transposition and improper PGBD5-dependent recombination. This suggested that the ability of PGBD5 to bind both pbles may not be based on the primary sequence of element ends, but may involve recognition of inner DNA motifs, possibly related to palindromic repeats. In agreement with this hypothesis, we identified internal palindromic repeats near the end of 24 pble sequences, which display distinct sequences. |
| Databáze: | OpenAIRE |
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