Endoglin is required for myogenic differentiation potential of neural crest stem cells

Autor: Teodora Nicola, Leif Oxburgh, Barbara A. Conley, Maria Cecilia Mancini, Douglas B. Spicer, Calvin P.H. Vary, Joseph M. Verdi
Rok vydání: 2007
Předmět:
Vascular smooth muscle
Smad Proteins
Muscle Development
Cardiovascular
Muscle
Smooth
Vascular
Mice
Neural crest
0302 clinical medicine
Cell Movement
hemic and lymphatic diseases
Cells
Cultured
Mice
Knockout
0303 health sciences
Myogenesis
Intracellular Signaling Peptides and Proteins
Endoglin
Gene Expression Regulation
Developmental
Cell biology
medicine.anatomical_structure
Stem cell
Signal Transduction
medicine.medical_specialty
Mice
Transgenic
Biology
Models
Biological
Article
03 medical and health sciences
Downregulation and upregulation
Internal medicine
otorhinolaryngologic diseases
medicine
Animals
Molecular Biology
Embryonic Stem Cells
DNA Primers
030304 developmental biology
Base Sequence
Neural tube
Cell Biology
Transforming growth factor beta
Rats
Mice
Inbred C57BL
Endocrinology
biology.protein
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Developmental Biology. 308:520-533
ISSN: 0012-1606
Popis: Genetic studies show that TGFbeta signaling is essential for vascular development, although the mechanism through which this pathway operates is incompletely understood. Here we demonstrate that the TGFbeta auxiliary coreceptor endoglin (eng, CD105) is expressed in a subset of neural crest stem cells (NCSCs) in vivo and is required for their myogenic differentiation. Overexpression of endoglin in the neural crest caused pericardial hemorrhaging, correlating with altered vascular smooth muscle cell investment in the walls of major vessels and upregulation of smooth muscle alpha-actin protein levels. Clonogenic differentiation assay of NCSCs derived from neural tube explants demonstrated that only NCSC expressing high levels of endoglin (NCSC(CD105+)) had myogenic differentiation potential. Furthermore, myogenic potential was deficient in NCSCs obtained from endoglin null embryos. Expression of endoglin in NCSCs declined with age, coinciding with a reduction in both smooth muscle differentiation potential and TGFbeta1 responsiveness. These findings demonstrate a cell autonomous role for endoglin in smooth muscle cell specification contributing to vascular integrity.
Databáze: OpenAIRE
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