Icariin Stimulates hFOB 1.19 Osteoblast Proliferation and Differentiation via OPG/RANKL Mediated by the Estrogen Receptor
| Autor: | Yu Guo, Lanqing Liao, Lin-Jun Sun, Zi-Fen Guo, Xiang-Hao Wen, Chong Li, Lu Guo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
musculoskeletal diseases
0106 biological sciences Pharmaceutical Science Estrogen receptor 01 natural sciences Collagen Type I Cell Line 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Osteogenesis 010608 biotechnology medicine Animals Humans RNA Messenger Cell Proliferation Flavonoids Osteoblasts biology Cell growth RANK Ligand Osteoprotegerin Osteoblast Cell Differentiation Alkaline Phosphatase medicine.anatomical_structure chemistry Receptors Estrogen RANKL 030220 oncology & carcinogenesis Cancer research Osteocalcin biology.protein Alkaline phosphatase Icariin Biotechnology |
| Zdroj: | Current pharmaceutical biotechnology. 22(1) |
| ISSN: | 1873-4316 |
| Popis: | Background:: Icariin (ICA), one of the main effective components isolated from the traditional Chinese herb Epimedium brevicornu Maxim., has been reported to possess extensive pharmacological actions, including enhanced sexual function, immune regulation, anti-inflammation, and antiosteoporosis. Methods:: Our study was designed to investigate the effect of ICA on cell proliferation and differentiation and the molecular mechanism of OPG/RANKL mediated by the Estrogen Receptor (ER) in hFOB1.19 human osteoblast cells. Results:: The experimental results show that ICA can stimulate cell proliferation and increase the activity of Alkaline Phosphatase (ALP), Osteocalcin (BGP) and I Collagen (Col I) and a number of calcified nodules. Furthermore, the mRNA and protein expression of OPG and RANKL and the OPG/ RANKL mRNA and protein expression ratios were upregulated by ICA. The above-mentioned results indicated that the optimal concentration of ICA for stimulating osteogenesis was 50ng/mL. Subsequent mechanistic studies comparing 50ng/mL ICA with an estrogen receptor antagonist demonstrated that the effect of the upregulated expression is connected with the estrogen receptor. In conclusion, ICA can regulate bone formation by promoting cell proliferation and differentiation and upregulating the OPG/RANKL expression ratio by the ER in hFOB1.19 human osteoblast cells. |
| Databáze: | OpenAIRE |
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