Human Exercise-Induced Circulating Progenitor Cell Mobilization Is Nitric Oxide-Dependent and Is Blunted in South Asian Men

Autor: Harry B. Rossiter, Daniel T. Cannon, Stephen B. Wheatcroft, Mark Rakobowchuk, Matthew Kahn, Karen M. Birch, Scott R. Murgatroyd, Adil Rajwani, Afroze Abbas, T. Scott Bowen, Karen E. Porter, Vivek Baliga, Richard M Cubbon, Carrie Ferguson, Mark T. Kearney
Rok vydání: 2010
Předmět:
Male
Brachial Artery
medicine.medical_treatment
CD34
Antigens
CD34
Vasodilation
Norepinephrine
chemistry.chemical_compound
Cell Movement
AC133 Antigen
Enzyme Inhibitors
Brachial artery
Infusions
Intravenous
Saline
Mobilization
Stem Cells
Cardiology and Cardiovascular Medicine
medicine.drug
Adult
Blood cells
medicine.medical_specialty
Down-Regulation
Hyperemia
macromolecular substances
Nitric Oxide
White People
Nitric oxide
Norepinephrine (medication)
Asian People
Antigens
CD
medicine.artery
Internal medicine
medicine
Humans
Endothelium
Progenitor cell
Exercise
Glycoproteins
omega-N-Methylarginine
business.industry
technology
industry
and agriculture
Endothelial Cells
Vascular Endothelial Growth Factor Receptor-2
Surgery
Endocrinology
chemistry
Endothelium
Vascular
Nitric Oxide Synthase
Peptides
business
Biomarkers
Zdroj: Arteriosclerosis, Thrombosis, and Vascular Biology. 30:878-884
ISSN: 1524-4636
1079-5642
DOI: 10.1161/atvbaha.109.201012
Popis: Objective— Circulating progenitor cells (CPC) have emerged as potential mediators of vascular repair. In experimental models, CPC mobilization is critically dependent on nitric oxide (NO). South Asian ethnicity is associated with reduced CPC. We assessed CPC mobilization in response to exercise in Asian men and examined the role of NO in CPC mobilization per se. Methods and Results— In 15 healthy, white European men and 15 matched South Asian men, CPC mobilization was assessed during moderate-intensity exercise. Brachial artery flow-mediated vasodilatation was used to assess NO bioavailability. To determine the role of NO in CPC mobilization, identical exercise studies were performed during intravenous separate infusions of saline, the NO synthase inhibitor l -NMMA, and norepinephrine. Flow-mediated vasodilatation (5.8%±0.4% vs 7.9%±0.5%; P =0.002) and CPC mobilization (CD34 + /KDR + 53.2% vs 85.4%; P =0.001; CD133 + /CD34 + /KDR + 48.4% vs 73.9%; P =0.05; and CD34 + /CD45 − 49.3% vs 78.4; P =0.006) was blunted in the South Asian group. CPC mobilization correlated with flow-mediated vasodilatation and l -NMMA significantly reduced exercise-induced CPC mobilization (CD34 + /KDR + −3.3% vs 68.4%; CD133 + /CD34 + /KDR + 0.7% vs 71.4%; and CD34 + /CD45 − −30.5% vs 77.8%; all P Conclusion— In humans, NO is critical for CPC mobilization in response to exercise. Reduced NO bioavailability may contribute to imbalance between vascular damage and repair mechanisms in South Asian men.
Databáze: OpenAIRE
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