Impact of Musashi-1 and Musashi-2 Double Knockdown on Notch Signaling and the Pathogenesis of Endometriosis
| Autor: | Theresa Strauß, Burkhard Greve, Michael Gabriel, Nurjannah Achmad, Dhanusha Schwan, Nancy Adriana Espinoza-Sanchez, Antonio Simone Laganà, Ludwig Kiesel, Matti Poutanen, Martin Götte, Sebastian Daniel Schäfer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Organic Chemistry
Medizin Endometriosis Nerve Tissue Proteins General Medicine Catalysis Computer Science Applications Inorganic Chemistry Necrosis Humans Female Physical and Theoretical Chemistry RNA Small Interfering Musashi Notch stem cells endometriosis apoptosis HES-1 KLF-4 Notch-2 SOX-2 ALDH Molecular Biology Spectroscopy Cell Proliferation Signal Transduction |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 5; Pages: 2851 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23052851 |
| Popis: | The stem cell marker and RNA-binding protein Musashi-1 is overexpressed in endometriosis. Musashi-1-siRNA knockdown in Ishikawa cells altered the expression of stem cell related genes, such as OCT-4. To investigate the role of both human Musashi homologues (MSI-1 and MSI-2) in the pathogenesis of endometriosis, immortalized endometriotic 12-Z cells and primary endometriotic stroma cells were treated with Musashi-1- and Musashi-2-siRNA. Subsequently, the impact on cell proliferation, cell apoptosis, cell necrosis, spheroid formation, stem cell phenotype and the Notch signaling pathway was studied in vitro. Using the ENDOMET Turku Endometriosis database, the gene expression of stem cell markers and Notch signaling pathway constituents were analyzed according to localization of the endometriosis lesions. The database analysis demonstrated that expression of Musashi and Notch pathway-related genes are dysregulated in patients with endometriosis. Musashi-1/2-double-knockdown increased apoptosis and necrosis and reduced stem cell gene expression, cell proliferation, and the formation of spheroids. Musashi silencing increased the expression of the anti-proliferation mediator p21. Our findings suggest the therapeutic potential of targeting the Musashi–Notch axis. We conclude that the Musashi genes have an impact on Notch signaling and the pathogenesis of endometriosis through the downregulation of proliferation, stemness characteristics and the upregulation of apoptosis, necrosis and of the cell cycle regulator p21. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |