Facilitating Imaging Mass Spectrometry of Microbial Specialized Metabolites with METASPACE
| Autor: | René De Mot, Veronika Saharuka, Lachlan Stuart, Theodore Alexandrov, Don D. Nguyen, Vittorio Venturi, Kinga Lubowiecka, Vitaly Kovalev, Massimo Del Prete |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Biochemistry & Molecular Biology Science & Technology 010405 organic chemistry Chemistry Communication Endocrinology Diabetes and Metabolism Metabolite Data interpretation Computational biology 01 natural sciences Biochemistry Microbiology imaging mass spectrometry Mass spectrometry imaging QR1-502 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound spatial metabolomics 030104 developmental biology microbial natural products Molecular Biology Life Sciences & Biomedicine |
| Zdroj: | Metabolites, Vol 11, Iss 477, p 477 (2021) Metabolites |
| Popis: | Metabolite annotation from imaging mass spectrometry (imaging MS) data is a difficult undertaking that is extremely resource intensive. Here, we adapted METASPACE, cloud software for imaging MS metabolite annotation and data interpretation, to quickly annotate microbial specialized metabolites from high-resolution and high-mass accuracy imaging MS data. Compared with manual ion image and MS1 annotation, METASPACE is faster and, with the appropriate database, more accurate. We applied it to data from microbial colonies grown on agar containing 10 diverse bacterial species and showed that METASPACE was able to annotate 53 ions corresponding to 32 different microbial metabolites. This demonstrates METASPACE to be a useful tool to annotate the chemistry and metabolic exchange factors found in microbial interactions, thereby elucidating the functions of these molecules. ispartof: METABOLITES vol:11 issue:8 ispartof: location:Switzerland status: published |
| Databáze: | OpenAIRE |
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