Transcriptomic-based bioassays for the detection of type A trichothecenes
| Autor: | Hanspeter Naegeli, Hans Gmuender, J. Stroka, P. Bowens, Katerina Lancova, T. Ellinger |
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| Přispěvatelé: | University of Zurich, Lancova, K |
| Rok vydání: | 2009 |
| Předmět: |
Tolerable daily intake
Fusarium Biology Toxicology medicine.disease_cause Microbiology Transcriptome chemistry.chemical_compound Gene expression medicine Bioassay Mycotoxin Gene 1106 Food Science Toxin Environmental and Occupational Health Public Health Environmental and Occupational Health 3005 Toxicology 10079 Institute of Veterinary Pharmacology and Toxicology 2739 Public Health Environmental and Occupational Health biology.organism_classification Molecular biology chemistry 570 Life sciences biology Public Health Food Science |
| DOI: | 10.5167/uzh-20192 |
| Popis: | The type A trichothecenes T-2 toxin (T-2) and HT-2 toxin (HT-2) are hazardous Fusarium products that contaminate many field crops growing in cold to temperate regions across the world. Toxicity studies in laboratory and farm animals have been used to derive a temporary tolerable daily intake (t-TDI) for the sum of T-2 and HT-2 of no more than 60 ng/kg body weight. To protect the consumers, it is now necessary to screen a large number of food samples for the presence of these poisonous fungal metabolites. Towards that goal, we discovered that the transcriptional apparatus of a human carcinoma cell line (MCF7) provides a sensitive biological sensor of type A trichothecenes. In fact, exposure of this easy-to-culture cell line to T-2 or HT-2 results in the regulation of >2,000 different transcripts with expression changes ranging from >5,000-fold gene inductions to >40-fold gene repressions. These transcriptional responses have been exploited to develop practical microchip and reverse transcription-polymerase chain reaction (RT-PCR) assays for the detection of type A trichothecenes at parts per billion levels. |
| Databáze: | OpenAIRE |
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