Molecular basis of Streptococcus mutans sortase A inhibition by the flavonoid natural product trans-chalcone
| Autor: | Jon Marles-Wright, Michael W. J. Dodds, Daynea Wallock-Richards, Bryan Hanley, Amarnath Maitra, Dominic J. Campopiano, David Clarke |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular Chalcone Mutant Crystallography X-Ray Catalysis Streptococcus mutans Cell wall chemistry.chemical_compound Bacterial Proteins Sortase Materials Chemistry Enzyme Inhibitors Biological Products Natural product Molecular Structure biology Chemistry Metals and Alloys Biofilm Stereoisomerism General Chemistry Aminoacyltransferases biology.organism_classification Surfaces Coatings and Films Electronic Optical and Magnetic Materials Cysteine Endopeptidases Biochemistry Sortase A Ceramics and Composites |
| Zdroj: | Wallock-Richards, D J, Marles-Wright, J, Clarke, D J, Maitra, A, Dodds, M, Hanley, B & Campopiano, D J 2015, ' Molecular basis of Streptococcus mutans sortase A inhibition by the flavonoid natural product trans-chalcone ', Chemical Communications, vol. 51, pp. 10483-10485 . https://doi.org/10.1039/c5cc01816a |
| DOI: | 10.1039/c5cc01816a |
| Popis: | Sortase A (SrtA) from Gram positive pathogens is an attractive target for inhibitors due to its role in the attachment of surface proteins to the cell wall. We found that the plant natural product trans-chalcone inhibits Streptococcus mutans SrtA in vitro and also inhibited S. mutans biofilm formation. Mass spectrometry revealed that the trans-chalcone forms a Michael addition adduct with the active site cysteine. The X-ray crystal structure of the SrtA H139A mutant provided new insights into substrate recognition by the sortase family. Our study suggests that chalcone flavonoids have potential as sortase-specific oral biofilm inhibitors. |
| Databáze: | OpenAIRE |
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