Identification of the infectious laryngotracheitis virus glycoprotein gB gene by the polymerase chain reaction
| Autor: | Jeffrey J. O'Brian, Calvin L. Keeler, Stuart J. Rabin, David J. Poulsen, Catherine R. Adams Burton |
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| Rok vydání: | 1991 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Chick Embryo Biology medicine.disease_cause Polymerase Chain Reaction Virus law.invention Open Reading Frames Restriction map Herpesvirus 1 Gallid Viral Envelope Proteins law Virology Sequence Homology Nucleic Acid Genetics medicine Animals Simplexvirus Amino Acid Sequence Molecular Biology Gene Polymerase chain reaction Base Sequence Nucleic acid sequence General Medicine Blotting Northern Molecular biology Open reading frame Herpes simplex virus DNA Probes |
| Zdroj: | Virus genes. 5(4) |
| ISSN: | 0920-8569 |
| Popis: | The infectious laryngotracheitis virus (ILTV) homologue of the herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) gene was identified by PCR amplification of genomic ILTV DNA. A 488-bp amplified DNA fragment was used to identify and clone two adjacent PstI fragments from genomic ILTV DNA. Sequence analysis of the region surrounding the amplified fragment identified a 2619-bp open reading frame that has 39% homology with both the nucleotide and amino-acid sequences of the HSV-1 gB gene. Northern blot analysis using a portion of the open reading frame as a probe identified a 2.7-kb RNA transcript in ILTV-infected chicken embryo liver cells. Analysis of the predicted amino acid sequence of the ILTV protein indicated that it shares structural features with the gB glycoproteins of other herpesviruses. |
| Databáze: | OpenAIRE |
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