Transmission of enteric non-A, non-B hepatitis virus in Macaca mulatta, monkeys by intraportal route: Subsequent passages of HEV virus
| Autor: | Yogendra Kumar Joshi, Tandon Bn, A. Varma, H.K. Gupta, B. Iyenger, S. Sriramchari, S. Shenoy |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
viruses
Biology Virus Serology Excretion Feces Hepatitis E virus medicine Animals Humans Microscopy Immunoelectron Hepatitis Hepatology Portal Vein Transmission (medicine) Virion Gastroenterology virus diseases medicine.disease Macaca mulatta Virology Hepatitis E Liver Injections Intravenous biology.protein Non b hepatitis Antibody Acute hepatitis |
| Zdroj: | Journal of Gastroenterology and Hepatology. 5:608-615 |
| ISSN: | 1440-1746 0815-9319 |
| DOI: | 10.1111/j.1440-1746.1990.tb01114.x |
| Popis: | Macaca mulatta monkeys have been used for the transmission of enteric non-A, non-B hepatitis (HEV) virus by intraportal route. Subsequent passages of HEV virus have been completed in these monkeys. In the first passage, 2 monkeys were inoculated by intra-portal route with 27–34 nm virus-like particles (VLP) obtained from known epidemics of HEV hepatitis in India, and biochemical and serological changes in the blood, histological changes in the liver and excretion of 27–34 nm VLP in the stool were studied. Results were compared with those of 4 negative control monkeys inoculated with stool extracts from healthy individuals. The second passage of 27–34 nm VLP was carried out on 2 monkeys using pools of stool suspension positive for 27–34 nm VLP from first passaged animals. Similarly, the third passage of 27–34 nm VLP was completed intraportally in another monkey. All monkeys developed acute hepatitis, as evidenced by transient elevation of aminotransferases, histopathological changes in the liver, development of antibodies aggregating 27–34 nm VLP and excretion of 27–34 nm VLP in stools. No control monkeys developed these features. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text