Hybridization kinetics of DNA fragments in the presence of ligands intercalating into DNA-duplexes

Autor:	V.B. Arakelyan, Poghos O. Vardevanyan, Aleksandr Y. Mamasakhlisov, Marine A. Parsadanyan, Eduard M. Kazaryan
Rok vydání:	2020
Předmět:	Dna duplex Stereochemistry Dissociation rate Kinetics Intercalation (chemistry) Nucleic Acid Hybridization DNA General Medicine Ligands chemistry.chemical_compound chemistry Structural Biology Molecule Molecular Biology
Zdroj:	Journal of Biomolecular Structure and Dynamics. 39:1907-1911
ISSN:	1538-0254 0739-1102
Popis:	In this work the hybridization kinetics of DNA fragments on underlayer has been studied, when besides single-stranded DNA molecules there are also ligands in the medium that are able to intercalate into DNA-duplexes. A system of differential equations was obtained that describes the correlative change of the number of DNA-duplexes on the underlayer and the change of the number of ligands intercalating into DNA-duplexes. It was shown that the rate of underlayer filling by DNA-duplexes increases along with the enhancement of both equilibrium constant of formation reaction of DNA-duplexes and the concentration of DNA targets in the solution. It was also shown that the intercalation kinetics of ligands into DNA-duplexes relevantly depends on relation of dissociation rate constant of DNA-duplex to dissociation rate of the ligand complex with DNA-duplex.Communicated by Ramaswamy H. Sarma.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c30330dd5ae196c6c1dbcf15cf0b9de5 https://doi.org/10.1080/07391102.2020.1739559 Zobrazit plný text záznamu