Preparation of Crystalline Cytochrome553 from Porphyra tenera

Autor:	Yasutomo Sugimura, Ichiro Morikawa, Kazuo Okunuki, Ichiro Sekuzu, Eijiro Yakushiji
Rok vydání:	1960
Předmět:	Porphyra chemistry.chemical_classification Multidisciplinary Chromatography Cytochrome biology Cytochrome c Eukaryota biology.organism_classification Column chromatography Enzyme Algae chemistry biology.protein Cytochromes Absorption (chemistry) Tenera
Zdroj:	Nature. 185:105-106
ISSN:	1476-4687 0028-0836
DOI:	10.1038/185105a0
Popis:	PARTIAL purification of a cytochrome component from Porphyra tenera, a component which has absorption peaks similar to that of cytochrome c or c 1 was first reported by Yakushiji1. Recently, Katoh and Takamiya (ref. 2 and Katoh, S., and Takamiya, A., personal communication) succeeded in obtaining a crystalline preparation of this component by means of diethylaminoethyl-cellulose column chromatography and reported the redox-potential of this and other components in marine algae. We also have crystallized the cytochrome by means of a cation-exchanger at pH 5.0. Since the diethylaminoethyl-cellulose column procedure is possible at pH 7.0, this procedure may be better; our method can be used to purify not only basic enzyme proteins, but also those that are stable in acidic pH.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c302792f9d47123631f10287815321ea https://doi.org/10.1038/185105a0 Zobrazit plný text záznamu