Evaluation of real-time PCR of patient pleural effusion for diagnosis of tuberculosis
| Autor: | Mirela Verza, Liliane Olival, Rosa Dea Sperhacke, Marcus Barreto Conde, Maria Lucia Rosa Rossetti, Arnaldo Zaha, Franciele Rosso, Candice Tosi Michelon, Renata Leborato Guerra |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
medicine.medical_specialty
Pathology Tuberculosis Pleural fluid specimen Pleural effusion Radiography Reação em cadeia da polimerase Short Report lcsh:Medicine General Biochemistry Genetics and Molecular Biology Positive predicative value medicine Tuberculose lcsh:Science (General) lcsh:QH301-705.5 Medicine(all) business.industry Biochemistry Genetics and Molecular Biology(all) lcsh:R General Medicine respiratory system medicine.disease respiratory tract diseases Real-time polymerase chain reaction lcsh:Biology (General) Pleural fluid Histopathology Radiology business Tempo real lcsh:Q1-390 |
| Zdroj: | BMC Research Notes Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS BMC Research Notes, Vol 4, Iss 1, p 279 (2011) |
| ISSN: | 1756-0500 |
| DOI: | 10.1186/1756-0500-4-279 |
| Popis: | Background Pleural tuberculosis (TB) diagnosis often requires invasive procedures such as pleural biopsy. The aim of this study was to evaluate the role of real-time polymerase chain reaction (PCR) for the IS 6110 sequence of M. tuberculosis in pleural fluid specimens as a rapid and non-invasive test for pleural TB diagnosis. Findings For this cross-sectional study, 150 consecutive patients with pleural effusion diagnosed by chest radiography, who were referred for diagnostic thoracocentesis and pleural biopsy and met eligibility criteria, had a pleural fluid specimen submitted for real-time PCR testing. Overall, 98 patients had pleural TB and 52 had pleural effusion secondary to other disease. TB diagnosis was obtained using acid-fast bacilli (AFB) smear or culture for mycobacteria and/or histopathologic examination in 94 cases and by clinical findings in 4 cases. Sensitivity, specificity, positive and negative predictive values of PCR testing for pleural TB diagnosis were 42.8% (95% CI 38.4 - 44.8), 94.2% (95% CI 85.8 - 98.0), 93.3% (95% CI 83.6 - 97.7), and 48.5% (95% CI 44.2 - 50.4), respectively. The real-time PCR test improved TB detection from 30.6% to 42.9% when compared to AFB smear and culture methods performed on pleural fluid specimens, although the best sensitivity was achieved by combining the results of culture and histopathology of pleural tissue specimens. Conclusion The real-time PCR test of pleural fluid specimens is a useful and non-invasive additional assay for fast diagnosis of pleural TB. |
| Databáze: | OpenAIRE |
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