Screening forEscherichia coliK-12 genes conferring glyoxal resistance or sensitivity by transposon insertions
| Autor: | Min-Suk Kwon, Nayoung Lee, Seong Hun Kim, Doyun Kim, Dong-Kyu Kim, Changhan Lee, Kihyun Lee, Chankyu Park, Jiyeun Kim, Haeyoung Min, Jihong Kim, Changmin Ko |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Transposable element TRNA modification Cyclic AMP Receptor Protein DNA repair Mutant Biology medicine.disease_cause Microbiology 03 medical and health sciences chemistry.chemical_compound RNA Transfer Transcription (biology) Drug Resistance Bacterial Genetics medicine Molecular Biology Escherichia coli Gene Escherichia coli K12 Escherichia coli Proteins Glyoxal Molecular biology Mutagenesis Insertional 030104 developmental biology chemistry Mutation DNA Transposable Elements DNA Adenylyl Cyclases |
| Zdroj: | FEMS Microbiology Letters. 363:fnw199 |
| ISSN: | 1574-6968 |
| DOI: | 10.1093/femsle/fnw199 |
| Popis: | Glyoxal (GO) belongs to the reactive electrophilic species generated in vivo in all organisms. In order to identify targets of GO and their response mechanisms, we attempted to screen for GO-sensitive mutants by random insertions of Tn phoA -132. The genes responsible for GO susceptibility were functionally classified as the following: (i) tRNA modification; trmE , gidA and truA , (ii) DNA repair; recA and recC , (iii) toxin–antitoxin; mqsA and (iv) redox metabolism; yqhD and caiC . In addition, an insertion in the crp gene, encoding the cAMP responsive transcription factor, exhibits a GO-resistant phenotype, which is consistent with the phenotype of adenylate cyclase ( cya ) mutant showing GO resistance. This suggests that global regulation involving cAMP is operated in a stress response to GO. To further characterize the CRP-regulated genes directly associated with GO resistance, we created double mutants deficient in both crp and one of the candidate genes including yqhD , gloA and sodB . The results indicate that these genes are negatively regulated by CRP as confirmed by real-time RT-PCR. We propose that tRNA as well as DNA are the targets of GO and that toxin/antitoxin, antioxidant and cAMP are involved in cellular response to GO. |
| Databáze: | OpenAIRE |
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