Selective expression of a splice variant of decay-accelerating factor in c-erbB-2-positive mammary carcinoma cells showing increased transendothelial invasiveness

Autor: Guido Sauter, Ronald Simon, Katrin Schier, Jan-Hendrik Mikesch, Burkhard Brandt, Dirk Kemming, Horst Bürger, Antje Rötger
Rok vydání: 2004
Předmět:
DNA
Complementary
Receptor
ErbB-2
Blotting
Western
Molecular Sequence Data
Biophysics
Breast Neoplasms
Biology
Immunofluorescence
Ligands
Biochemistry
Polymerase Chain Reaction
Receptors
G-Protein-Coupled
Western blot
Antigens
CD
Cell Movement
medicine
Biomarkers
Tumor
Humans
Neoplasm Invasiveness
RNA
Messenger
Cloning
Molecular
Receptor
Molecular Biology
Decay-accelerating factor
In Situ Hybridization
Oligonucleotide Array Sequence Analysis
Electrophoresis
Agar Gel
Complement Inactivator Proteins
Membrane Glycoproteins
medicine.diagnostic_test
Base Sequence
CD55 Antigens
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Alternative splicing
Cell migration
Cell Biology
Complement System Proteins
Molecular biology
Immunohistochemistry
In vitro
Gene Expression Regulation
Neoplastic
Alternative Splicing
Microscopy
Fluorescence
Cell culture
RNA
Endothelium
Vascular
Zdroj: Biochemical and biophysical research communications. 329(1)
ISSN: 0006-291X
Popis: By differential-display-PCR a subclone of the SK-BR-3 cell line with high in vitro transendothelial invasiveness was identified to express increased levels of a new alternative splice variant of decay-accelerating factor (DAF). DAF seems to play an important role in some malignant tumours since on the one hand the expression of complement inhibitors on the surface of tumour cells prevents the accumulation of complement factors and in consequence cell lysis. On the other hand, DAF has been identified as a ligand for the CD97 surface receptor which induces cell migration. Immunofluorescence procedures, Western blot analyses, and cDNA clone sequencing were employed to confirm the expression of DAF restricted to invasive tumour cells. Using a radioactive RNA-in situ hybridisation on freshly frozen tissue microarrays and RT-PCR on native tumour tissue, the expression of alternative spliced DAF mRNA was demonstrated in invasive breast cancer. Due to the fact that it could thereby not be detected in normal mammary tissues, it has to be confirmed in larger studies that the DAF splice variant might be a specific tumour marker for invasive breast cancer.
Databáze: OpenAIRE
Externí odkaz: